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Product Name
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GFAP antibody [G-A-5] (Cy3 ®)
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See all GFAP antibodies (29)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [G-A-5] to GFAP (Cy3 ®)
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Immunogen
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Purified GFAP from pig spinal cord
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Reacts with
(species key)
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Hu, Rat, Cat, Chk, Gfsh, Pig
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Specificity
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Recognizes an epitope localized on the carboxy terminal Cys II fragment of GFAP and the N-terminal part of the tail sequence of the molecule.
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Tested applications
(see key)
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IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/400.
The epitope recognized by the antibody is partially sensitive to prolonged formalin fixation, but is resistant to alcohol-fixation followed by paraffin-embedding and to acetone-fixation of frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Rat cerebellum.
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Cellular localization
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Cytoplasmic
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Research areas
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Stem Cells >> Neural Stem Cells >> Glial Restricted Lineage >> Astrocyte
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class II >> GFAP
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
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Relevance
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Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
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GFAP antibody [G-A-5] (Cy3 ®) (ab49874) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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G-A-5
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Isotype
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IgG1
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Purity
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IgG fraction
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Conjugation notes
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Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) conjugated to Cy3. The Cy3-antibody conjugate is extensively dialyzed to remove unbound Cy3.
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Conjugation
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Cy3 ®
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Concentration
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1.200 mg/ml
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Storage instructions
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Store at +4°C in the dark. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GFAP antibody [G-A-5] (Cy3 ®) is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to GFAP
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GFAP antibody [G-A-5] (Cy3 ®) - more information
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Customer reviews (feedback) regarding GFAP antibody [G-A-5] (Cy3 ®) |
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Protocols for GFAP antibody [G-A-5] (Cy3 ®) |
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Contact Abcam with a Technical Enquiry about GFAP antibody [G-A-5] (Cy3 ®) |
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