GFAP antibody [GA-5] - BSA and Azide free (ab80842)

Product Name 

GFAP antibody [GA-5] - BSA and Azide free

Product type 

Primary antibodies

Description 

Mouse monoclonal [GA-5] to GFAP - BSA and Azide free

Immunogen 

Pig spinal cord.

Reacts with 
(species key)

Hu, Rat, Chk, Pig

Specificity 

ab80842 stains astrocytes, glial cells, ependymal cells and their corresponding tumors. Many types of neural tumors such as neuroblastomas, Schwannomas, as well as extra CNS tumors are not labeled.

Tested applications 
(see key)

ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 1 - 2 µg/ml (for 30 min at RT).
IP: Use at 2 µg/mg of lysate. (Native and denatured, use Protein G).
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

IMR5 cells, brain and astrocytoma tissue.

Cellular localization 

Cytoplasmic

Research areas 

Stem Cells >> Neural Stem Cells >> Glial Restricted Lineage >> Astrocyte
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class II >> GFAP
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments

Relevance 

Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

GA-5

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: None
Constituents: 10mM PBS, pH 7.4

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for GFAP antibody [GA-5] - BSA and Azide free images, references, products related to ab80842 and other tools.

GFAP antibody [GA-5] - BSA and Azide free images:

Customer reviews (feedback) regarding GFAP antibody [GA-5] - BSA and Azide free

Customer FAQs regarding GFAP antibody [GA-5] - BSA and Azide free

Protocols for GFAP antibody [GA-5] - BSA and Azide free

Price and availability of products related to GFAP antibody [GA-5] - BSA and Azide free

GFAP antibody [GA-5] - BSA and Azide free - related products: