Specific protocols
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To our knowledge, customised protocols are not required for this product.
Please try the standard protocols listed below and let us know how you get on. |
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General protocols
Useful resources:Western blotting (WB) protocols:Immunohistochemistry (IHC) / Immunocytochemistry (ICC) protocols:Chromatin Immunoprecipitation (ChIP) protocols:Dot blot protocols:ELISA protocols:ELISPOT protocols: Flow cytometry / FACS protocols:Immunoprecipitation (IP) protocols: |
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunocytochemistry - GFAP antibody [GF5] - Astrocyte Marker (ab10062)](/ps/datasheet/Images/10/ab10062/ab10062_1.jpg)
Immunocytochemistry - GFAP antibody [GF5] - Astrocyte Marker (ab10062)
Mouse monoclonal to GFAP [GF5] (ab10062) was used in fixed rat glial cultures at a dilution of 1/100, and incubated overnight at 4°C. Alexa Fluor 568 (1/400) secondary goat anti-mouse antibody was used for detection. Fluoresence microscopy revealed diffuse cytosolic labelling. Counterstaining with TO-PRO-3 (Molecular Probes; 660nm (converted here to blue colour) was used to identify the nucleus. The “fibrous” anti-GFAP staining of murine mixed cultures is typical of what is expected.
Randal Moldrich, CNRS UMR7637, ESPCI, France
![Immunocytochemistry - GFAP antibody [GF5] - Astrocyte Marker (ab10062)](/ps/datasheet/Images/10/ab10062/ab10062_2.jpg)
Immunocytochemistry - GFAP antibody [GF5] - Astrocyte Marker (ab10062)
Mouse monoclonal to GFAP [GF5] (ab10062) was used in fixed murine cultures (mixed neurons/glia) at 1/100 overnight at 4°C. A secondary goat anti-mouse antibody was used for detection (Alexa Fluor 568; 1/400). Microscopy revealed diffuse cytosolic labelling. Coounterstaining with TO-PRO-3 (Molecular Probes; 660nm (converted here to blue colour) was used to identify the nucleus. The “fibrous” anti-GFAP staining of murine mixed cultures is typical of what is expected.
Randal Moldrich, CNRS UMR7637, ESPCI, France
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFAP antibody [GF5] - Astrocyte Marker (ab10062)](/ps/datasheet/Images/10/ab10062/ab10062_id9172_reduced.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFAP antibody [GF5] - Astrocyte Marker (ab10062)
GFAP antibody [GF5] - Astrocyte Marker (ab10062; 1/250 for 16h) used in Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) on Rat Tissue sections (adult brain: lateral ventricle showing astrocytes).Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Secondary Antibody: biotin labelled goat anti mouse Ig (1/200). This image shows the exit point for the progenitor olfactory neurones, of the lateral ventrical subventricular zone.
Carl Hobbs, CARD, KCL, UK
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFAP antibody [GF5] - Astrocyte Marker (ab10062)](/ps/datasheet/images/10/ab10062/GFAP-Primary-antibodies-ab10062-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFAP antibody [GF5] - Astrocyte Marker (ab10062)
GFAP antibody [GF5] - Astrocyte Marker (ab10062) immunocytochemical detection in stimulated Cor1 cells. Stimulated Cor1 cells were fixed in formaldehyde, permeabilized, blocked in 1% BSA for 10 mins @ rt°C. Primary Antibody ab10062 incubated at 1/1500 for 2 hours in TBS/BSA/azide/0.3% triton. Secondary Antibody: anti mouse IgG Conjugated to: Alexa Fluor® 488 (1/1000).
This image is courtesy of an Abreview submitted by Mr Carl Hobbs
![GFAP antibody [GF5] - Astrocyte Marker for IHC-Fr in Rat (10062)](/ps/Reviews/Images/ab8375_36670.jpg)
GFAP antibody [GF5] - Astrocyte Marker for IHC-Fr in Rat (10062)
![GFAP antibody [GF5] - Astrocyte Marker for in Mouse (10062)](/ps/Reviews/Images/ab3892_10877.jpg)
GFAP antibody [GF5] - Astrocyte Marker for in Mouse (10062)
![GFAP antibody [GF5] - Astrocyte Marker for ICC/IF in Mouse (10062)](/ps/datasheet/images/10/ab10062/GFAP-Primary-antibodies-ab10062-1.jpg)
GFAP antibody [GF5] - Astrocyte Marker for ICC/IF in Mouse (10062)
![Anti-GFAP antibody [GF5] - Astrocyte Marker for Immunocytochemistry/ Immunofluorescence in Human (10062)](/ps/datasheet/images/10/ab10062/GFAP-Primary-antibodies-ab10062-23.jpg)
Anti-GFAP antibody [GF5] - Astrocyte Marker for Immunocytochemistry/ Immunofluorescence in Human (10062)
![Anti-GFAP antibody [GF5] - Astrocyte Marker for Immunocytochemistry/ Immunofluorescence in Rat (10062)](/ps/datasheet/images/10/ab10062/GFAP-Primary-antibodies-ab10062-34.jpg)
Anti-GFAP antibody [GF5] - Astrocyte Marker for Immunocytochemistry/ Immunofluorescence in Rat (10062)
![GFAP antibody [GF5] - Astrocyte Marker for IHC-P in Rat (10062)](/ps/Reviews/Images/ab9172_44455.jpg)
GFAP antibody [GF5] - Astrocyte Marker for IHC-P in Rat (10062)
![GFAP antibody [GF5] - Astrocyte Marker for IHC-P in Zebrafish (10062)](/ps/Reviews/Images/ab9202_45760.jpg)
GFAP antibody [GF5] - Astrocyte Marker for IHC-P in Zebrafish (10062)
![GFAP antibody [GF5] - Astrocyte Marker for in Rat (10062)](/ps/Reviews/Images/ab3891_10876.jpg)
GFAP antibody [GF5] - Astrocyte Marker for in Rat (10062)
![Anti-GFAP antibody [GF5] - Astrocyte Marker for Immunocytochemistry/ Immunofluorescence in Rat (10062)](/ps/datasheet/images/10/ab10062/GFAP-Primary-antibodies-ab10062-17.jpg)
Anti-GFAP antibody [GF5] - Astrocyte Marker for Immunocytochemistry/ Immunofluorescence in Rat (10062)
![Anti-GFAP antibody [GF5] - Astrocyte Marker for Immunocytochemistry/ Immunofluorescence in Human (10062)](/ps/datasheet/images/10/ab10062/GFAP-Primary-antibodies-ab10062-30.jpg)
Anti-GFAP antibody [GF5] - Astrocyte Marker for Immunocytochemistry/ Immunofluorescence in Human (10062)
![Anti-GFAP antibody [GF5] - Astrocyte Marker for Immunocytochemistry/ Immunofluorescence in Human (10062)](/ps/datasheet/images/10/ab10062/GFAP-Primary-antibodies-ab10062-9.jpg)
Anti-GFAP antibody [GF5] - Astrocyte Marker for Immunocytochemistry/ Immunofluorescence in Human (10062)
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