Products:Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
If your product does not perform as described on this datasheet, we will refund or replace your product...
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Thank you soooo much, the antibodys worked and the student finished in time for the presentation. I can not thank you enough. |
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ANSWER: |
I am glad it all worked out for you and the student. |
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No staining in IHC-P using rat samples. |
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ANSWER: |
Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. |
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No staining in mouse and rat brain tissue, despite troubleshooting. |
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ANSWER: |
Thanks for your call today and for letting us know about the trouble with ab1316 and ab10062.
As we discussed, I am sending vials of ab4648 and ab68334 free of charge on the order ***. These should arrive tomorrow.
Please keep me updated of the results using these replacement antibodies, and let me know if there is anything else that we can do for you. |
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I am currently considering purchasing your Mouse Anti-GFAP Monoclonal Antibody, Unconjugated for an ELISA. I was wondering if you could send me information regarding its specificity, rate of recovery and minimal detectable limits. Thanks in advance for your assistance.
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ANSWER: |
Thank you for your enquiry. I am sorry it has taken some time to find some information for you. As far as I am aware, this antibody (ab10062) has been tested in ELISA but unfortunately, there is no data available on the minimal detectable limits or specificity. I am sorry I am unable to help you further. Please get back in touch if you have any other questions, and I will be happy to help. |
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I am going to purchase two antibodies for immunofluorescence imaging and would like to find suitable isotype controls for them. The antibodies I am going to purchase are: ab18460 and ab10062. Do you carry isotypes for these antibodies? |
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ANSWER: |
Thank you for your enquiry. The correct isotype control for ab10062 is ab18428, while the correct isotype control for ab18460 is ab18449. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Mouse monoclonal to GFAP [GF5] (ab10062) was used in fixed rat glial cultures at a dilution of 1/100, and incubated overnight at 4°C. Alexa Fluor 568 (1/400) secondary goat anti-mouse antibody was used for detection. Fluoresence microscopy revealed diffuse cytosolic labelling. Counterstaining with TO-PRO-3 (Molecular Probes; 660nm (converted here to blue colour) was used to identify the nucleus. The “fibrous” anti-GFAP staining of murine mixed cultures is typical of what is expected.
Randal Moldrich, CNRS UMR7637, ESPCI, France
Mouse monoclonal to GFAP [GF5] (ab10062) was used in fixed murine cultures (mixed neurons/glia) at 1/100 overnight at 4°C. A secondary goat anti-mouse antibody was used for detection (Alexa Fluor 568; 1/400). Microscopy revealed diffuse cytosolic labelling. Coounterstaining with TO-PRO-3 (Molecular Probes; 660nm (converted here to blue colour) was used to identify the nucleus. The “fibrous” anti-GFAP staining of murine mixed cultures is typical of what is expected.
Randal Moldrich, CNRS UMR7637, ESPCI, France
GFAP antibody [GF5] - Astrocyte Marker (ab10062; 1/250 for 16h) used in Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) on Rat Tissue sections (adult brain: lateral ventricle showing astrocytes).Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Secondary Antibody: biotin labelled goat anti mouse Ig (1/200). This image shows the exit point for the progenitor olfactory neurones, of the lateral ventrical subventricular zone.
Carl Hobbs, CARD, KCL, UK
GFAP antibody [GF5] - Astrocyte Marker (ab10062) immunocytochemical detection in stimulated Cor1 cells. Stimulated Cor1 cells were fixed in formaldehyde, permeabilized, blocked in 1% BSA for 10 mins @ rt°C. Primary Antibody ab10062 incubated at 1/1500 for 2 hours in TBS/BSA/azide/0.3% triton. Secondary Antibody: anti mouse IgG Conjugated to: Alexa Fluor® 488 (1/1000).
This image is courtesy of an Abreview submitted by Mr Carl Hobbs
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