Anti-GFAP antibody [GF5] - Astrocyte Marker (ab10062)

Overview

• Product nameAnti-GFAP antibody [GF5] - Astrocyte MarkerSee all GFAP primary antibodies ...
• Description
  Mouse monoclonal [GF5] to GFAP - Astrocyte Marker
• SpecificityThere is no cross-reactivity with other neurospecific proteins.
• Tested applicationsIHC-P, WB, ELISA, IHC-FoFr, IHC-Fr, ICC/IF, Flow Cyt, ICC more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  This clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunised with purified glial fibrillary acidic protein from human brain.

• Positive controlnormal adult rat brain: lateral ventricle
• General notesConcentration varies from lot to lot and can be provided on request.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: PBS, pH 7.4
• Concentration information loading...
• PurityProtein G purified
• Purification notesPurity is tested by electrophoresis.
• Clonality Monoclonal
• Clone numberGF5
• IsotypeIgG2b
• Research Areas
  • Stem Cells
  • Neural Stem Cells
  • Glial Restricted Lineage
  • Astrocyte

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Intermediate Filaments
  • Class II
  • GFAP

  • Tags & Cell Markers
  • Cell Type Markers
  • Neuroscience Markers
  • Glial

  • Neuroscience
  • Cell Adhesion Proteins
  • Cytoskeletal Proteins
  • Intermediate Filaments

  • Neuroscience
  • Cell Type Marker
  • Glia marker
  • Astrocyte marker

Applications

Target

• FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
• Tissue specificityExpressed in cells lacking fibronectin.
• Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
• Sequence similaritiesBelongs to the intermediate filament family.
• Post-translational
  modificationsPhosphorylated by PKN1.
• Cellular localizationCytoplasm. Associated with intermediate filaments.

Target information above from: UniProt accession P14136 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2670 Human
  • Entrez Gene: 14580 Mouse
  • Entrez Gene: 24387 Rat
  • Omim: 137780 Human
  • SwissProt: P14136 Human
  • SwissProt: P03995 Mouse
  • SwissProt: P47819 Rat
  • Unigene: 514227 Human

  • Unigene: 1239 Mouse
  • Unigene: 91512 Rat

  see all

• Alternative names
  Astrocyte antibodyFLJ42474 antibodyFLJ45472 antibody

  GFAP antibodyGFAP_HUMAN antibodyGlial Fibrillary Acidic Protein antibodyIntermediate filament protein antibody

  see all

Anti-GFAP antibody [GF5] - Astrocyte Marker images

• 

  Immunocytochemistry - GFAP antibody [GF5] - Astrocyte Marker (ab10062)Randal Moldrich, CNRS UMR7637, ESPCI, France

  Mouse monoclonal to GFAP [GF5] (ab10062) was used in fixed rat glial cultures at a dilution of 1/100, and incubated overnight at 4°C. Alexa Fluor 568 (1/400) secondary goat anti-mouse antibody was used for detection. Fluoresence microscopy revealed diffuse cytosolic labelling. Counterstaining with TO-PRO-3 (Molecular Probes; 660nm (converted here to blue colour) was used to identify the nucleus. The “fibrous” anti-GFAP staining of murine mixed cultures is typical of what is expected.

• 

  Immunocytochemistry - GFAP antibody [GF5] - Astrocyte Marker (ab10062)Randal Moldrich, CNRS UMR7637, ESPCI, France

  Mouse monoclonal to GFAP [GF5] (ab10062) was used in fixed murine cultures (mixed neurons/glia) at 1/100 overnight at 4°C. A secondary goat anti-mouse antibody was used for detection (Alexa Fluor 568; 1/400). Microscopy revealed diffuse cytosolic labelling. Coounterstaining with TO-PRO-3 (Molecular Probes; 660nm (converted here to blue colour) was used to identify the nucleus. The “fibrous” anti-GFAP staining of murine mixed cultures is typical of what is expected.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFAP antibody [GF5] - Astrocyte Marker (ab10062)Carl Hobbs, CARD, KCL, UK
  GFAP antibody [GF5] - Astrocyte Marker (ab10062; 1/250 for 16h) used in Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) on Rat Tissue sections (adult brain: lateral ventricle showing astrocytes).Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Secondary Antibody: biotin labelled goat anti mouse Ig (1/200). This image shows the exit point for the progenitor olfactory neurones, of the lateral ventrical subventricular zone.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFAP antibody [GF5] - Astrocyte Marker (ab10062)This image is courtesy of an Abreview submitted by Mr Carl Hobbs
  GFAP antibody [GF5] - Astrocyte Marker (ab10062) immunocytochemical detection in stimulated Cor1 cells. Stimulated Cor1 cells were fixed in formaldehyde, permeabilized, blocked in 1% BSA for 10 mins @ rt°C. Primary Antibody ab10062 incubated at 1/1500 for 2 hours in TBS/BSA/azide/0.3% triton. Secondary Antibody: anti mouse IgG Conjugated to: Alexa Fluor® 488 (1/1000).

  See Abreview

• 

  Flow Cytometry-Anti-GFAP antibody [GF5] - Astrocyte Marker(ab10062)
  Overlay histogram showing SH-SY5Y cells stained with ab10062 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10062, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.