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Anti-GFAP antibody [GF5] - Astrocyte Marker (ab10062)

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Overview

Product name

Anti-GFAP antibody [GF5] - Astrocyte Marker
See all GFAP products (35) ...

Description

Mouse monoclonal [GF5] to GFAP - Astrocyte Marker

Specificity

There is no cross-reactivity with other neurospecific proteins.

Tested applications

IHC-P, WB, ELISA, IF, IHC-FoFr, IHC-Fr, ICC/IF, ICCmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

This clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunised with purified glial fibrillary acidic protein from human brain.

Positive control

normal adult rat brain: lateral ventricle

General notes

Concentration varies from lot to lot and can be provided on request.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

Purity is tested by electrophoresis.

Clonality

Monoclonal

Clone number

GF5

Isotype

IgG2b

Research areas

Stem Cells >> Neural Stem Cells >> Glial Restricted Lineage >> Astrocyte
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class II >> GFAP
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments

  • Immunocytochemistry - GFAP antibody [GF5] - Astrocyte Marker (ab10062)Immunocytochemistry - GFAP antibody [GF5] - Astrocyte Marker (ab10062) image (enlarge)

  • Immunocytochemistry - GFAP antibody [GF5] - Astrocyte Marker (ab10062)Immunocytochemistry - GFAP antibody [GF5] - Astrocyte Marker (ab10062) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFAP antibody [GF5] - Astrocyte Marker (ab10062)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFAP antibody [GF5] - Astrocyte Marker (ab10062) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab10062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Tissue specificity

Expressed in cells lacking fibronectin.

Involvement in disease

Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.

Sequence similarities

Belongs to the intermediate filament family.

Post-translational
modifications

Phosphorylated by PKN1.

Cellular localization

Cytoplasm. Associated with intermediate filaments.

Target information above from: UniProt accessionP14136 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Astrocyte antibody
  • FLJ42474 antibody
  • FLJ45472 antibody
  • GFAP antibody
  • GFAP_HUMAN antibody
  • Glial Fibrillary Acidic Protein antibody
  • Intermediate filament protein antibody
see all

Database links

see all

Anti-GFAP antibody [GF5] - Astrocyte Marker images:

  Immunocytochemistry - GFAP antibody [GF5] - Astrocyte Marker (ab10062)

Immunocytochemistry - GFAP antibody [GF5] - Astrocyte Marker (ab10062)

Mouse monoclonal to GFAP [GF5] (ab10062) was used in fixed rat glial cultures at a dilution of 1/100, and incubated overnight at 4°C. Alexa Fluor 568 (1/400) secondary goat anti-mouse antibody was used for detection. Fluoresence microscopy revealed diffuse cytosolic labelling. Counterstaining with TO-PRO-3 (Molecular Probes; 660nm (converted here to blue colour) was used to identify the nucleus. The “fibrous” anti-GFAP staining of murine mixed cultures is typical of what is expected.

Randal Moldrich, CNRS UMR7637, ESPCI, France

  Immunocytochemistry - GFAP antibody [GF5] - Astrocyte Marker (ab10062)

Immunocytochemistry - GFAP antibody [GF5] - Astrocyte Marker (ab10062)

Mouse monoclonal to GFAP [GF5] (ab10062) was used in fixed murine cultures (mixed neurons/glia) at 1/100 overnight at 4°C. A secondary goat anti-mouse antibody was used for detection (Alexa Fluor 568; 1/400). Microscopy revealed diffuse cytosolic labelling. Coounterstaining with TO-PRO-3 (Molecular Probes; 660nm (converted here to blue colour) was used to identify the nucleus. The “fibrous” anti-GFAP staining of murine mixed cultures is typical of what is expected.

Randal Moldrich, CNRS UMR7637, ESPCI, France

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFAP antibody [GF5] - Astrocyte Marker (ab10062)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFAP antibody [GF5] - Astrocyte Marker (ab10062)

GFAP antibody [GF5] - Astrocyte Marker (ab10062; 1/250 for 16h) used in Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) on Rat Tissue sections (adult brain: lateral ventricle showing astrocytes).Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Secondary Antibody: biotin labelled goat anti mouse Ig (1/200). This image shows the exit point for the progenitor olfactory neurones, of the lateral ventrical subventricular zone.

Carl Hobbs, CARD, KCL, UK

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFAP antibody [GF5] - Astrocyte Marker (ab10062)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFAP antibody [GF5] - Astrocyte Marker (ab10062)

GFAP antibody [GF5] - Astrocyte Marker (ab10062) immunocytochemical detection in stimulated Cor1 cells. Stimulated Cor1 cells were fixed in formaldehyde, permeabilized, blocked in 1% BSA for 10 mins @ rt°C. Primary Antibody ab10062 incubated at 1/1500 for 2 hours in TBS/BSA/azide/0.3% triton. Secondary Antibody: anti mouse IgG Conjugated to: Alexa Fluor® 488 (1/1000).

This image is courtesy of an Abreview submitted by Mr Carl Hobbs

See Abreview

References for Anti-GFAP antibody [GF5] - Astrocyte Marker (ab10062)

This product has been referenced in:

  • Braun BJet al. The formyl peptide receptor like-1 and scavenger receptor MARCO are involved in glial cell activation in bacterial meningitis. J Neuroinflammation 8:11 (2011). ICC/IF; Rat.Read more (PubMed: 21299846) »
  • Shah Aet al. HIV-1 envelope protein gp120 up regulates CCL5 production in astrocytes which can be circumvented by inhibitors of NF-?B pathway. Biochem Biophys Res Commun : (2011). ICC/IF; Human.Read more (PubMed: 21945445) »

See all 6 publications for this product

Publishing research using ab10062? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"