Anti-GFAP antibody [GF5] - Astrocyte Marker (ab10062)
- Product nameAnti-GFAP antibody [GF5] - Astrocyte MarkerSee all GFAP primary antibodies ...
- DescriptionMouse monoclonal [GF5] to GFAP - Astrocyte Marker
- SpecificityThere is no cross-reactivity with other neurospecific proteins.
- Tested applicationsIHC-P, WB, ELISA, IHC-FoFr, IHC-Fr, ICC/IF, Flow Cyt, ICC more details
- Species reactivityReacts with: Mouse, Rat, Human
This clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunised with purified glial fibrillary acidic protein from human brain.
- Positive controlnormal adult rat brain: lateral ventricle
- General notesConcentration varies from lot to lot and can be provided on request.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
- Concentration information loading...
- PurityProtein G purified
- Purification notesPurity is tested by electrophoresis.
- Clonality Monoclonal
- Clone numberGF5
Our Abpromise guarantee covers the use of ab10062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||IHC-P: 1/250 - 1/1500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||WB: Use at an assay dependent dilution. Detects a band of approximately 43-45 kDa.|
|ELISA||ELISA: Use at an assay dependent dilution.|
|IHC-FoFr||IHC-FoFr: Use at an assay dependent dilution. PubMed: 20708681|
|ICC/IF||ICC/IF: Use at an assay dependent dilution.|
|Flow Cyt||Flow Cyt: Use 1-2µg for 106 cells.|
- FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
- Tissue specificityExpressed in cells lacking fibronectin.
- Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
- Sequence similaritiesBelongs to the intermediate filament family.
modificationsPhosphorylated by PKN1.
- Cellular localizationCytoplasm. Associated with intermediate filaments.
- Astrocyte antibodyFLJ42474 antibodyFLJ45472 antibody
- GFAP antibodyGFAP_HUMAN antibodyGlial Fibrillary Acidic Protein antibodyIntermediate filament protein antibody
Anti-GFAP antibody [GF5] - Astrocyte Marker images
Mouse monoclonal to GFAP [GF5] (ab10062) was used in fixed rat glial cultures at a dilution of 1/100, and incubated overnight at 4°C. Alexa Fluor 568 (1/400) secondary goat anti-mouse antibody was used for detection. Fluoresence microscopy revealed diffuse cytosolic labelling. Counterstaining with TO-PRO-3 (Molecular Probes; 660nm (converted here to blue colour) was used to identify the nucleus. The “fibrous” anti-GFAP staining of murine mixed cultures is typical of what is expected.
Mouse monoclonal to GFAP [GF5] (ab10062) was used in fixed murine cultures (mixed neurons/glia) at 1/100 overnight at 4°C. A secondary goat anti-mouse antibody was used for detection (Alexa Fluor 568; 1/400). Microscopy revealed diffuse cytosolic labelling. Coounterstaining with TO-PRO-3 (Molecular Probes; 660nm (converted here to blue colour) was used to identify the nucleus. The “fibrous” anti-GFAP staining of murine mixed cultures is typical of what is expected.
GFAP antibody [GF5] - Astrocyte Marker (ab10062; 1/250 for 16h) used in Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) on Rat Tissue sections (adult brain: lateral ventricle showing astrocytes).Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Secondary Antibody: biotin labelled goat anti mouse Ig (1/200). This image shows the exit point for the progenitor olfactory neurones, of the lateral ventrical subventricular zone.
GFAP antibody [GF5] - Astrocyte Marker (ab10062) immunocytochemical detection in stimulated Cor1 cells. Stimulated Cor1 cells were fixed in formaldehyde, permeabilized, blocked in 1% BSA for 10 mins @ rt°C. Primary Antibody ab10062 incubated at 1/1500 for 2 hours in TBS/BSA/azide/0.3% triton. Secondary Antibody: anti mouse IgG Conjugated to: Alexa Fluor® 488 (1/1000).
Overlay histogram showing SH-SY5Y cells stained with ab10062 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10062, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-GFAP antibody [GF5] - Astrocyte Marker (ab10062)
This product has been referenced in:
- Huang JF et al. Differential neuronal expression of receptor interacting protein 3 in rat retina: involvement in ischemic stress response. BMC Neurosci 14:16 (2013). IHC-FoFr ; Rat . Read more (PubMed: 23374330) »
- Slowik A et al. Involvement of formyl peptide receptors in receptor for advanced glycation end products (RAGE)--and amyloid beta 1-42-induced signal transduction in glial cells. Mol Neurodegener 7:55 (2012). ICC/IF ; Rat . Read more (PubMed: 23164356) »