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Product name
Anti-GFI1 antibody
See all GFI1 products (4) ...
Description
Rabbit polyclonal to GFI1
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Cow, Human
Immunogen
Synthetic peptide, corresponding to internal sequence amino acids 280-330 of Human GFI1 (Q99684).
Positive control
HepG2 and RAW cell lysates, Human brain tissue
Properties
Form
Liquid
Storage instructions
Store at 4°C (up to 6 months). For long term storage store at -20°C
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab107589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 6 - 8 µg/ml. Predicted molecular weight: 45 kDa.
IHC-P: Use a concentration of 5 µg/ml.
Target
Function
Transcription factor regulating the expression of genes active during S-phase of the cell cycle in hematopoietic cells. Represses ELA2 transcription.
Involvement in disease
Defects in GFI1 are a cause of neutropenia severe congenital autosomal dominant type 2 (SCN2) [MIM:613107]. SCN2 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
Defects in GFI1 are a cause of dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA) [MIM:607847]. NI-CINA is a relatively mild form of neutropenia diagnosed in adults, but predisposing to leukemia in a subset of patients.
Sequence similarities
Contains 6 C2H2-type zinc fingers.
Domain
The Gly/Ala-rich region may indicate that Gfi-1 is a repressor of its target genes.
Post-translational
modifications
Ubiquitinated.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ99684
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- GFI 1 antibody
- GFI1 antibody
- GFI1_HUMAN antibody
see all
Database links
- Entrez Gene: 2672 Human
- Entrez Gene: 14581 Mouse
- Entrez Gene: 24388 Rat
- Omim: 600871 Human
- SwissProt: Q99684 Human
- SwissProt: Q8C287 Mouse
- SwissProt: P70338 Mouse
- SwissProt: Q07120 Rat
see all
Anti-GFI1 antibody images:
Western blot - GFI1 antibody (ab107589)
All lanes : Anti-GFI1 antibody (ab107589) at 6 µg/ml
Lane 1 : Human HepG2 cell lysate
Lane 2 : Human HepG2 cell lysate with Immunizing peptide
Lane 3 : Mouse RAW cell lysate
Secondary
Goat anti-rabbit HRP
developed using the ECL technique
Predicted band size : 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFI1 antibody (ab107589)
Immunohistochemical analysis of GFI1 in formalin-fixed, paraffin-embedded human brain tissue using ab107589 at 5 µg/ml.
References for Anti-GFI1 antibody (ab107589)
ab107589 has not yet been referenced specifically in any publications.
Publishing research using ab107589? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"