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Immunocytochemistry/ Immunofluorescence - GFP antibody (FITC) (ab6662)
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Immunohistochemistry (Frozen sections) - GFP antibody (FITC) (ab6662)
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Product Name
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GFP antibody (FITC)
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See all GFP antibodies (26)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to GFP (FITC)
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Immunogen
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Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
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Specificity
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This GFP antibody will cross react with YFP, CFP, eGFP, but not RFP.
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Tested applications
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ELISA, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC/IF: 1/200 - 1/400.
IHC-P: Use at an assay dependent dilution (PMID 18446241).
IHC-Fr: Use at an assay dependent dilution.
IHC-FoFr: 1/250.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Tags & Cell Markers >> Fusion / Marker Proteins >> GFP
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Relevance
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Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.
Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation. Fluorescein conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts.
Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone.
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Conjugation notes
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Fluorescein isothiocyanate (FITC) (MW 390 daltons)
Absorption Wavelength: 495 nm
Emission Wavelength: 528 nm
Fluorochrome/Protein Ratio: 3.5 moles FITC per mole of Goat IgG
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Form
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Liquid
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Conjugation
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Fluorescein
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GFP antibody (FITC) is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GFP antibody (FITC) images, references, products related to ab6662 and other tools.
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GFP antibody (FITC) images:
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 Immunocytochemistry/ Immunofluorescence - GFP antibody (FITC) (ab6662)
These pictures show confocal immunofluorescence using GFP-expressing glial cells (green) transplanted into the lesioned rat spinal cord. This was detected using ab6662 and a standard FITC filter set. Axons are labelled red by an antibody to neurofilament-200 and a rhodamine secondary antibody. The upper panel shows the centre of the transplant site at low power. Numerous GFP-positive cells can be seen mingling with axons. The lower panel shows, at high power in a single optical section, how ab6662 reveals the morphology of the transplanted cells to such an extent that their close interactions with axons are obvious - the cell depicted can be seen wrapping around a neurofilament-200 positive axon.
These images were kindly supplied as part of the review submitted by Andrew Toft.
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Immunohistochemistry (Frozen sections) - GFP antibody (FITC) (ab6662)
ab6662 staining mouse brain tissue sections (inducible GFP reporter) by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum and then incubated with the antibody at a 1/1000 dilution for 1 hour. Staining is shown in the left hand panel. The middle panel shows staining with a rabbit anti-GFP antibody and the right hand panel shows the merged images (plus DAPI). ab6662 gives no noticable background and it is found that when viewing on an epifluorescent the exposure time is significantly reduced. This image is courtesy of an Abreview submitted by Dr Joshua Breunig
See Abreview
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References for GFP antibody (FITC) (ab6662)
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This product has been used in: (two most recent references)
Kim KK et al. Epithelial cell alpha3beta1 integrin links beta-catenin and Smad signaling to promote myofibroblast formation and pulmonary fibrosis. J Clin Invest 119:213-24 (2009). WB. PubMed: 19104148
Lombaert IM et al. Rescue of salivary gland function after stem cell transplantation in irradiated glands. PLoS ONE 3:e2063 (2008). IHC-P. PubMed: 18446241
See all 7 publication references for this product.
If you publish research using ab6662 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to GFP
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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