GFP antibody [GFP01 (B34)] (ab3277)

Product Name 

GFP antibody [GFP01 (B34)]

Product type 

Primary antibodies

Description 

Mouse monoclonal [GFP01 (B34)] to GFP

Immunogen 

Purified recombinant GFP protein.

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IP: Undiluted. Please see abreview for further details.
WB: Use at an assay dependent concentration (from PubMed:16867977)

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Purified recombinant GFP protein, transfected cell lines expressing GFP tagged proteins.

Research areas 

Tags & Cell Markers >> Fusion / Marker Proteins >> GFP

Relevance 

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

GFP01 (B34)

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

Notes 

Molecular weight of antigen: 27 kDa.

See below for GFP antibody [GFP01 (B34)] images, references, products related to ab3277 and other tools.

GFP antibody [GFP01 (B34)] images:

References for GFP antibody [GFP01 (B34)] (ab3277)

  This product has been used in: (two most recent references)

Nagle CA et al. Hepatic overexpression of glycerol-sn-3-phosphate acyltransferase 1 in rats causes insulin resistance. J Biol Chem 282:14807-15 (2007). PubMed: 17389595

Drenan RM et al. R7BP augments the function of RGS7*Gbeta5 complexes by a plasma membrane-targeting mechanism. J Biol Chem 281:28222-31 (2006). PubMed: 16867977

See all 3 publication references for this product.


If you publish research using ab3277 please let us know so that we can cite the reference on this datasheet.

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