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Anti-GFP antibody (HRP) (ab6663)

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Overview

Product name

Anti-GFP antibody (HRP)
See all GFP products (30) ...

Description

Goat polyclonal to GFP (HRP)

Conjugation

HRP

Conjugation notes

Horseradish Peroxidase (HRP)

Specificity

Antibody recognizes wild type, recombinant and enhanced forms of GFP.

Tested applications

WB, IP, ELISA, Dot Blotmore details

Immunogen

The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

pH: 7.20
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 50% Glycerol, 0.42% Potassium phosphate, 0.87% Sodium chloride

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Primary antibody notes

Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation Peroxidase conjugated anti-GFP assayed by immunoblot shows a 42 kDa band when reacted with GFP on a western blot.

Clonality

Polyclonal

Isotype

IgG

Research areas

Tags & Cell Markers >> Fusion / Marker Proteins >> GFP

Applications

Show applications key

Our Abpromise guarantee covers the use of ab6663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.

Alternative names

  • GFP antibody
  • Green Fluorescent Protein antibody

References for Anti-GFP antibody (HRP) (ab6663)

This product has been referenced in:

  • Osabe M & Negishi M Active ERK1/2 Protein Interacts with the Phosphorylated Nuclear Constitutive Active/Androstane Receptor (CAR; NR1I3), Repressing Dephosphorylation and Sequestering CAR in the Cytoplasm. J Biol Chem 286:35763-9 (2011). WB.Read more (PubMed: 21873423) »
  • Kunde SA  et al. The X-chromosome-linked intellectual disability protein PQBP1 is a component of neuronal RNA granules and regulates the appearance of stress granules. Hum Mol Genet 20:4916-31 (2011).Read more (PubMed: 21933836) »

See all 10 publications for this product

Publishing research using ab6663? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"