If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please let me know ab1214 can use detect enhanced GFP sample |
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ANSWER: |
Thank you for contacting us. |
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Just wanted to clarify one point - I have submitted my review for the antibody below, and wanted to make sure that the code was active now, even though I have not heard anything back yet regarding my review. Thank you for your time, and have a great day! |
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ANSWER: |
Thank you for your reply. |
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I would like to test ab1218 for both WB and IP of eYFP. Once I get the discount code from you, I will go ahead and make the purchase. Thanks for all the help, and I hope you have/had a nice Easter weekend. |
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ANSWER: |
I am very pleased to hear you would like to accept our offer and test ab1218 in eYFP. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for eYFP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. |
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I think that we would be interested in that offer, but I had one question – Would we still be able to return the antibody for a refund if it does not work? Thanks for the help, and I look forward to hearing from you. |
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ANSWER: |
Thank you very much for your interest in ab1218. |
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WIll these antibodies recognize eYFP (enhance YFP) or mCherry? |
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ANSWER: |
Thank you for your inquiry. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunoprecipitation using ab1218 at 0.01µl/µl HEK (human embryonic kidney) 293 whole cell lysate incubated with protein G matrix for 1 hour at 4°C.
Total protein input was 3,000,000 cells.
Lane 1: GFP-RhoA wt
Lane 2: GFP-Rac1 wt
Lane 3: GFP-empty
The western blot antibody was ab290 (undiluted).
This image is courtesy of an Abreview submitted by Dr Peter Jordan
ab1218 staining GFP in human prostate epithelial primary cells by Immunocytochemistry/ Immunofluorescence. Cells (ARPE-19 cells transfected with mouse Rab27A-GFP) were fixed with paraformaldehyde, permeabilized with 0.5% Triton x100 and blocking with 5% serum was performed for 20 minutes at 25°C. Samples were incubated with primary antibody (1/500: in 1% goat serum, 0.1%TX100, 1XPBS) for 16 hours at 4°C. An Alexa Fluor®546-conjugated goat polyclonal to mouse IgG was used as secondary antibody at 1/500 dilution. DAPI was used to stain the cell nuclei (blue) and mRab27A-GFP with ab1218 (red).
This image is a courtesy of Vladimir Milenkovic
Anti-GFP antibody (ab1218) at 1/1000 dilution + Human HEK293 whole cell lysate at 3 µg
Secondary
A Pacific BlueTM-conjugated Goat anti-mouse IgG Monoclonal at 1/3000 dilution
developed using the ECL technique
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
Exposure time : 40 seconds
Blocking STep: 5% Milk for 45 minutes at 24°C
GST fusion protein ectopically expressed in HEK293 cells.
This image is courtesy of an anonymous Abreview
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