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ab13970 has been referenced in 47 publications.
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Transgenic mice expressing GFP selectively in lamina II of the spinal cord. In the right panels, note the correspondance between the green (rabbit anti-GFP) and red signals (chicken anti-GFP from Abcam) indicating that these two antibody preparations recognized the same gene product. The secondary antibody used with ab13970 was a FITC-labeled goat anti-chicken
Western blot of transgenic mouse spinal cords showing that the rabbit anti-GFP (lane 1) and the chicken anti-GFP (Abcam; lane 2) recognize a band at the same molecular weight.
ab13970 staining mouse olfactory epithelium tissue sections by IHC-Fr. The sample was PFA fixed and blocked with 4% BSA/ 5% NFDM/ 10% NDS for 15 minutes at 20°C. The primary antibody was diluted 1/1000 and incubated with the sample for 1 hour. A FITC conjugated goat anti-chicken was used as the secondary.
This colony is the result of retroviral infection with a control virus. The GFP is under the control of an IRES promoter, so its expression is independent of any other protein. The counter-stain is hoescht.
This image is courtesy of an Abreview submitted by Dr Schwob Lab
ab13970 staining mouse olfactory bulb tissue sections by IHC-Fr. Sections were PFA fixed, permeabilized in 0.4% Triton-X and blocked with 5% serum for 2 hours at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 488 conjugated goat anti-chicken was used as the secondary.
This image is courtesy of an anonymous Abreview
ab13970 staining GFP + tumor in mouse muscle cells by ICC/IF. Cells were formaldehyde fixed and blocked with 3% BSA for 1 hour at 24°C prior to incubation with the primary antibody (1/500) for 1 hour at 24°C. An Alexa Fluor® 488 conjugated goat anti-chicken was used as the secondary.
This image is courtesy of an Abreview submitted by Dr Radbod Darabi
All lanes : Anti-GFP antibody (ab13970) at 1/500 dilution
Lane 1 : Mouse mammary carcinoma cell line clone expressing GFP - whole cell lysate
Lane 2 : Mouse mammary carcinoma cell line clone expressing GFP - whole cell lysate
Lane 3 : Mouse mammary carcinoma cell line clone expressing GFP - whole cell lysate
Lane 4 : Mouse mammary carcinoma cell line clone expressing GFP - whole cell lysate
Lane 5 : Mouse mammary carcinoma cell line clone expressing GFP - whole cell lysate
Lane 6 : Parental cell line of mouse mammary carcinoma clones, not expressing GFP - whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP conjugated goat anti-chicken at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 27-30 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
Results from several clones of mouse mammary carcinoma cell line which express GFP (left) and the parental cell line which does not express GFP (far right). This chicken antibody detects GFP in different forms which may represent species specific modifications (see review with human cells). Beta-actin (~47 kD) is shown as loading control.
This image is courtesy of an anonymous Abreview
ab13970 staining GFP in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed with paraformaldehyde and permeabilized with 0.1% Triton X100 before blocking with 10% serum for 30 minutes at 250C. The sample was incubated with primary antibody (1/2000) for 16 hours at 250C in 10% NGS in PBS + 0.1% TX100. An Alexa Fluor®488-conjugated Goat polyclonal to chicken IgG was used as secondary antibody at 1/400 dilution. In the image, green staining represents GFP expressed in oligodendrocytes, blue is for ToPro3.
This image is a courtesy of Ben Deverman
ab13970 staining GFP in murine lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then permeabilized with 0.1% Tween, blocked with 15% serum for 30 minutes at 23°C and then incubated with ab13970 at a 1/500 dilution for 14 hours at 4 °C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-chicken polyclonal used at a 1/500 dilution.
Image courtesy of an anonymous Abreview.
All lanes : Anti-GFP antibody (ab13970) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from HeLa cells.
Lane 2 : Whole cell lysate prepared from HeLa cells.
Lysates/proteins at 25 µg per lane.
Secondary
IRDye 800CW conjugated goat anti-chicken polyclonal at 1/15000 dilution
Image courtesy of an anonymous Abreview.
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