Immunofluorescence - GFP antibody (ab6556)

This image shows IF using GFP-expressing glial cells (green) transplanted into lesioned rat spinal cord. This was detected using ab6556 anti-GFP antibody and a FITC conjugated secondary antibody. Axons are labelled red by an antibody to neurofilament-200 and a rhodamine secondary. ab6556 reveals the morphology of the transplanted cells to such an extent that their close interactions with axons are obvious. The top picture shows an optical section from a confocal microscope scan showing how a GFP cell wraps around a branched axon travelling longitudinally. The bottom picture consists of an optical section from another confocal scan showing a GFP cell enveloping an axon in the transverse plane. Review by Andrew Toft submitted 19 May 2004

Electron Microscopy - GFP antibody (ab6556)

Specific labeling of a Trk-GFP fusion protein being synthesized on ER in sympathetic neurons infected with an adenovirus carrying the construct. The gold is associated with the ER membranes. This was done using a 1:5000 dilution of affinity purified antibody (ab6556). The tissue section was fixed and embedded using durcupan resin.

Immunohistochemistry (Frozen sections) - GFP antibody (ab6556)

ab6556 staining GFP in mouse tooth tissue section by Immunohistochemistry (Frozen sections) without fixation. Tissue samples were blocked with 1% BSA for 20 minutes at 20⁰C. The sample was incubated with primary antibody (1/500) for 2 hours at 20⁰C. An Alexa Fluor^®488-conjugated Chicken polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution. Immunofluorescent localization of CD31 and GFP in implanted non GFP cultured molars in GFP adult mice showed the origin of neo-formed blood vessels.
DP: Dental Pulp
EO: Enamel Organ

This image is a courtesy of Adnane Nait Lechguer

Immunohistochemistry (Frozen sections) - GFP antibody (ab6556)

ab6556 at 1/500 dilution staining GFP in mouse testis by immunohistochemistry (frozen sections). Sections were paraformaldehyde fixed prior to blocking in 100% serum for 1 hour at 37ºC and then incubated with ab6556 for 1 hour at 37ºC. A Texas Red conjugated chicken polyclonal to rabbit Ig, diluted 1/100, was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

- GFP antibody (ab6556)

This image shows a single primary hippocampal neuron from a primary culture overexpressing GFP stained with ab6556 at a dilution of 1:2000. This picture was kindly supplied as part of the review submitted by one of our customers.