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Products:Tags & Cell Markers >> Fusion / Marker Proteins >> GFP
Overview
Product name
Anti-GFP antibody
See all GFP products (29) ...
Description
Rabbit polyclonal to GFP
Specificity
This antibody is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP.
Tested applications
IHC-FoFr, Electron Microscopy, IHC-P, IHC-Fr, IP, WB, ICC/IF, Flow Cytmore details
Immunogen
Highly purified recombinant full length GFP made in Escherichia coli
Positive control
pure GFP protein, or cells known to overexpress GFP.
General notes
Please note that a mistake was made in reference 4 (Mesaeli et.al., J. Cell. Biol. 1999 Mar 8;144(5):857-68). The antibody used for immunohistochemistry on paraformaldehyde fixed tissues was the crude serum version of this antibody (Abcam ab290) and not Clontech's monoclonal as stated.
This product is supplied in 25% glycerol. During freezeing and thawing some phase separation might occur - Please ensure that the solution is mixed thoroughly but GENTLY before use.
This product should be refrigerated at all times.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 25% Glycerol, 50mM Tris HCl. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody is an affinity purified rabbit anti-GFP antibody purified on an affinity chromatography column made with highly purified recombinant GFP.
Primary antibody notes
This antibody (ab6556) is the purifed version of our best-selling rabbit polyclonal to GFP, ab290. It has been developed specifically for use in applications requiring a high titre and specificity with minimum background such as immuno-electron microscopy.
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 19563657)
EM: 1/5000
IHC-P: 1/1000Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-Fr: 1/1000
IP: Use at an assay dependent dilution. (Use Protein A agarose for IP.)
WB: 1/1000 - 1/5000.
ICC/IF: 1/2000
-
Flow Cyt: Use at an assay dependent dilution.
Target
Relevance
Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.
Alternative names
- GFP antibody
- Green Fluorescent Protein antibody
Anti-GFP antibody images:
Immunofluorescence - GFP antibody (ab6556)
This image shows IF using GFP-expressing glial cells (green) transplanted into lesioned rat spinal cord. This was detected using ab6556 anti-GFP antibody and a FITC conjugated secondary antibody. Axons are labelled red by an antibody to neurofilament-200 and a rhodamine secondary. ab6556 reveals the morphology of the transplanted cells to such an extent that their close interactions with axons are obvious. The top picture shows an optical section from a confocal microscope scan showing how a GFP cell wraps around a branched axon travelling longitudinally. The bottom picture consists of an optical section from another confocal scan showing a GFP cell enveloping an axon in the transverse plane. Review by Andrew Toft submitted 19 May 2004
Electron Microscopy - GFP antibody (ab6556)
Specific labeling of a Trk-GFP fusion protein being synthesized on ER in sympathetic neurons infected with an adenovirus carrying the construct. The gold is associated with the ER membranes. This was done using a 1:5000 dilution of affinity purified antibody (ab6556). The tissue section was fixed and embedded using durcupan resin.
Immunohistochemistry (Frozen sections) - GFP antibody (ab6556)
ab6556 staining GFP in mouse tooth tissue section by Immunohistochemistry (Frozen sections) without fixation. Tissue samples were blocked with 1% BSA for 20 minutes at 200C. The sample was incubated with primary antibody (1/500) for 2 hours at 200C. An Alexa Fluor®488-conjugated Chicken polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution. Immunofluorescent localization of CD31 and GFP in implanted non GFP cultured molars in GFP adult mice showed the origin of neo-formed blood vessels.
DP: Dental Pulp
EO: Enamel Organ
This image is a courtesy of Adnane Nait Lechguer
Immunohistochemistry (Frozen sections) - GFP antibody (ab6556)
ab6556 at 1/500 dilution staining GFP in mouse testis by immunohistochemistry (frozen sections). Sections were paraformaldehyde fixed prior to blocking in 100% serum for 1 hour at 37°C and then incubated with ab6556 for 1 hour at 37°C. A Texas Red conjugated chicken polyclonal to rabbit Ig, diluted 1/100, was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
- GFP antibody (ab6556)
This image shows a single primary hippocampal neuron from a primary culture overexpressing GFP stained with ab6556 at a dilution of 1:2000. This picture was kindly supplied as part of the review submitted by one of our customers.
References for Anti-GFP antibody (ab6556)
This product has been referenced in:
- Sheridan JTet al. Characterization of the oligomeric structure of the Ca(2+)-activated Cl- channel Ano1/TMEM16A. J Biol Chem 286:1381-8 (2011). WB.Read more (PubMed: 21056985) »
- Alm-Kristiansen AHet al. PIAS1 interacts with FLASH and enhances its co-activation of c-Myb. Mol Cancer 10:21 (2011). WB.Read more (PubMed: 21338522) »
See all 116 publications for this product
Publishing research using ab6556? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"