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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-GFPT2 antibody
See all GFPT2 products (2) ...
Description
Mouse polyclonal to GFPT2
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
GFPT2 (AAH00012.1, 1 a.a. ~ 682 a.a) full-length human protein.
Positive control
GFPT2 transfected 293T lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Energy Metabolism
Signal Transduction >> Metabolism >> Amino Acids
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 10 µg/ml
WB: 1/500 - 1/1000.Detects a band of approximately 75 kDa (predicted molecular weight: 77 kDa).
Target
Relevance
GFPT2, also known as glutamine fructose 6 phosphate transaminase 2, controls the flux of glucose into the hexosamine pathway. It is probably involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins.
Alternative names
- D fructose 6 phosphate amidotransferase 2 antibody
- GFAT 2 antibody
- GFAT2 antibody
see all
Database links
- Entrez Gene: 9945 Human
- GenBank: BC000012.2 Human
- Omim: 603865 Human
- SwissProt: O94808 Human
Anti-GFPT2 antibody images:
Western blot - GFPT2 antibody (ab70078)
All lanes : Anti-GFPT2 antibody (ab70078) at 1/500 dilution
Lane 1 : GFPT2 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 77 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence-GFPT2 antibody(ab70078)
ICC/IF image of ab70078 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70078, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-GFPT2 antibody (ab70078)
ab70078 has not yet been referenced specifically in any publications.
Publishing research using ab70078? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"