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Anti-GFR alpha 3 antibody (ab6171)

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1 question for ab6171

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Question 1

Monday 06-November-2000

Dear Supplier Could you tell me the fixative protocol for your new antisera GDNF alpha2 and GDNF alpha3 receptors?

ANSWER:

 

The protocol prefered for these antibodies (both alpha 2 and 3) uses a preperfusion of 1% sodium nitrite in phosphate buffer, followed by PBS then PFA (4%) containing 0.15% satd. picric acid.

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