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Anti-GFR alpha 3 antibody (ab8028)

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Overview

Product name

Anti-GFR alpha 3 antibody
See all GFR alpha 3 products (3) ...

Description

Rabbit polyclonal to GFR alpha 3

Tested applications

IHC (PFA fixed), IHC-P, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide, corresponding to amino acids 347-360 of Mouse GFR alpha 3.

Positive control

Murine kidney, liver or heart tissue lysates.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

IgG fraction

Primary antibody notes

Members of the glial cell line-derived neurotrophic factor(GDNF) family including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRa and Ret receptor tyrosine kinase GFRb. Following the findings of GFRa-1 and -2, a novel receptor in GFRa family was identified very recently from human and mouse and designated GFRa-3. GFRa-3 binds persephin, thus, persephin, GFRa-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function.

Clonality

Polyclonal

Isotype

IgG

Research areas

Neuroscience >> Neurology process >> Growth and Development >> Neurotrophins

  • Western blot - GFR alpha 3 antibody (ab8028)Western blot - GFR alpha 3 antibody (ab8028) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFR alpha 3 antibody (ab8028)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFR alpha 3 antibody (ab8028) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab8028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IHC-P: Use at a concentration of 10 µg/ml.
Immunohistochemistry (PFA fixed): 1/50.
WB: 1/500 - 1/1000. Predicted molecular weight: 43 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase.

Tissue specificity

Widely expressed in adult and fetus which exhibit a similar pattern. Essentially not expressed in the central nervous system, but highly expressed in several sensory and sympathetic ganglia of the peripheral nervous system. Moderate expression in many non-neuronal tissues, particularly those of the digestive and urogenital systems, but high expression in stomach and appendix. Several types of glandular tissues show low expression. Very low or no expression detected in the hematopoietic system.

Sequence similarities

Belongs to the GDNFR family.

Post-translational
modifications

N-glycosylated.

Cellular localization

Cell membrane.

Target information above from: UniProt accessionO60609 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • GDNF family receptor alpha 3 antibody
  • GDNF family receptor alpha-3 antibody
  • GDNF receptor alpha-3 antibody
  • GDNFR-alpha-3 antibody
  • GFR-alpha-3 antibody
  • GFRA3 antibody
  • GFRA3_HUMAN antibody
  • Glial cell line derived neurotrophic factor receptor alpha 3 antibody
  • GPI linked receptor antibody
  • OTTHUMP00000159486 antibody
  • OTTHUMP00000224020 antibody
see all

Database links

see all

Anti-GFR alpha 3 antibody images:

  Western blot - GFR alpha 3 antibody (ab8028)

Western blot - GFR alpha 3 antibody (ab8028)

All lanes : Anti-GFR alpha 3 antibody (ab8028) at 1/500 dilution

Lane 1 : Crude cell membrane fractions of murine heart
Lane 2 : Crude cell membrane fractions of murine spleen
Lane 3 : Crude cell membrane fractions of murine kidney
Lane 4 : Crude cell membrane fractions of murine liver
Lane 5 : Crude cell membrane fractions of murine brain


Predicted band size : 43 kDa
Observed band size : 43 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFR alpha 3 antibody (ab8028)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFR alpha 3 antibody (ab8028)

ab8028 at 10µg/ml staining GFR alpha 3 in mouse heart tissue by IHC-P.

References for Anti-GFR alpha 3 antibody (ab8028)

This product has been referenced in:

  • Ceyhan GOet al. The neurotrophic factor artemin promotes pancreatic cancer invasion. Ann Surg 244:274-81 (2006). WB, IHC-P; Human.Read more (PubMed: 16858191) »
  • Serra MPet al. Ret, GFRalpha-1, GFRalpha-2 and GFRalpha-3 receptors in the human hippocampus and fascia dentata. Int J Dev Neurosci 23:425-38 (2005).Read more (PubMed: 16002253) »

See all 2 publications for this product

Publishing research using ab8028? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"