Anti-GGA2 antibody (ab10552)

Hi, I'm looking for a good GGA2 antibody for ICC in rabbit cultured cells or IHC in mouse tissue. I saw the reference on your page to a reasonably good ICC picture for Cos7 cells and thought it might be worthwhile to try in our cells- but was wondering: what is the sequence homology between the human peptide sequence used for this antibody and the mouse GGA2? Do you think there would be a good chance for positive results? Thanks.

ANSWER:

Thank you for your enquiry.

As far as we are aware, cross reactivity with mouse has not yet been tested for use with ab10552. All species cross-reactivity information is stated on Abcam product datasheets, and these are updated as soon as any new information is brought to our attention.

However, I have run a BLAST search (results below) using the immunogen sequence and found that there is 82% homolgy with mouse. We would normally expect a high chance of positive results if the homology was more than 90%. However, this does not mean that the antibody will not work, just that we have not tested it and therefore, can not guarantee that you will get good results.

GENE ID: 74105 Gga2 | golgi associated, gamma adaptin ear containing, ARF binding protein 2 [Mus musculus] (Over 10 PubMed links)

Score = 46.4 bits (102), Expect = 6e-06 Identities = 14/17 (82%), Positives = 15/17 (88%), Gaps = 0/17 (0%)

Query 1 LSMYRRPGQAPPDQEAL 17 LSMYRRPG A PDQ+AL Sbjct 2 LSMYRRPGHALPDQQAL 18

If you decide to go ahead and purchase this product, we encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers.

To find out more about our Abreview system, please see the following URL: http://www.abcam.com/abreviews

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.