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- GGA2 antibody (ab10552)
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Product Name
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GGA2 antibody
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See all GGA2 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to GGA2
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Immunogen
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Synthetic peptide: LSMYRRPGQAPPDQEAL conjugated to KLH, corresponding to amino acids 249-265 of Human GGA2.
BLAST 'LSMYRRPGQAPPDQEAL' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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This antibody recognises two bands by Western at 75 and 37kD. This corresponds to results seen in Hela extracts in Dell'Angelica et al. 2000. The lower 37kD band is expected to correspond to the isoform described in NP_619581.
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Tested applications
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ICC/IF, WB
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
WB: 1/500. Detects a band of approximately 75 & 37 kDa (predicted molecular weight: 67 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein.
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Research areas
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Signal Transduction >> Protein Trafficking >> Organelle Proteins
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Relevance
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GGA2 is a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain that mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.400 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GGA2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GGA2 antibody images, references, products related to ab10552 and other tools.
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GGA2 antibody images:
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 - GGA2 antibody (ab10552)
Predicted band size : 67 kDa
Western blot using ab10552 at 1/500. Lysates at 5µg per lane. Secondary ab6721 at 1/5000.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: Jurkat
Lane 5: 293
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References for GGA2 antibody (ab10552)
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This product has been used in:
Lefranois S & McCormick PJ The Arf GEF GBF1 is required for GGA recruitment to Golgi membranes. Traffic 8:1440-51 (2007). ICC/IF. PubMed: 17666033
If you publish research using ab10552 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to GGA2
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GGA2 antibody - more information
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Customer reviews (feedback) regarding GGA2 antibody |
Customer FAQs regarding GGA2 antibody |
Protocols for GGA2 antibody |
Price and availability of products related to GGA2 antibody |
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GGA2 antibody - related products:
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GGA2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about GGA2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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