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Products:Signal Transduction >> Protein Trafficking >> Organelle Proteins
Overview
Product name
Anti-GGA2 antibody
See all GGA2 products (3) ...
Description
Rabbit polyclonal to GGA2
Specificity
This antibody recognises two bands by Western at 75 and 37kD. This corresponds to results seen in Hela extracts in Dell'Angelica et al. 2000. The lower 37kD band is expected to correspond to the isoform described in NP_619581.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide: LSMYRRPGQAPPDQEAL conjugated to KLH, corresponding to amino acids 249-265 of Human GGA2.
LSMYRRPGQA PPDQEAL
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Organelle Proteins
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Western blot - Anti-GGA2 antibody (ab10552)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
WB: 1/500Detects a band of approximately 75 , 37 kDa (predicted molecular weight: 67 kDa).
Target
Function
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain.
Domain
The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function.
Post-translational
modifications
Ubiquitinated.
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
Target information above from: UniProt accessionQ9UJY4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ARF-binding protein 2 antibody
- gamma ear-containing antibody
- 1200007E24Rik antibody
see all
Database links
- Entrez Gene: 23062 Human
- Entrez Gene: 74105 Mouse
- NCBI: 20336264 Human
- SwissProt: Q9UJY4 Human
- SwissProt: Q6P5E6 Mouse
- Unigene: 133340 Human
- Unigene: 460336 Human
- Unigene: 29619 Mouse
Anti-GGA2 antibody images:
Western blot - Anti-GGA2 antibody (ab10552)
Predicted band size : 67 kDa
Western blot using ab10552 at 1/500. Lysates at 5µg per lane. Secondary ab6721 at 1/5000.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: Jurkat
Lane 5: 293
References for Anti-GGA2 antibody (ab10552)
This product has been referenced in:
- Lefrançois S & McCormick PJ The Arf GEF GBF1 is required for GGA recruitment to Golgi membranes. Traffic 8:1440-51 (2007). ICC/IF.Read more (PubMed: 17666033) »
See 1 publication for this product
Publishing research using ab10552? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"