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Products:Signal Transduction >> Protein Trafficking >> Golgi Proteins
Overview
Product name
Anti-GGA3 antibody - Aminoterminal end
See all GGA3 products (6) ...
Description
Rabbit polyclonal to GGA3 - Aminoterminal end
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the N-terminal region of human GGA3, conjugated to KLH
Positive control
293 cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Golgi Proteins
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Western blot - GGA3 antibody (ab59957)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
Target
Function
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain.
Domain
The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function.
Post-translational
modifications
Phosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
Ubiquitinated.
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
Target information above from: UniProt accessionQ9NZ52
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ARF-binding protein 3 antibody
- gamma ear-containing antibody
- ADP ribosylation factor binding protein 3 antibody
see all
Database links
- Entrez Gene: 23163 Human
- Omim: 606006 Human
- SwissProt: Q9NZ52 Human
- Unigene: 87726 Human
Anti-GGA3 antibody - Aminoterminal end images:
Western blot - GGA3 antibody (ab59957)
Anti-GGA3 antibody - Aminoterminal end (ab59957) at 1/50 dilution + 293 cell lysate at 35 µg
Predicted band size : 78 kDa
Observed band size : 78 kDa
References for Anti-GGA3 antibody - Aminoterminal end (ab59957)
ab59957 has not yet been referenced specifically in any publications.
Publishing research using ab59957? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"