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Western blot - GGA3 antibody (ab10553)
Western blot - GGA3 antibody (ab10553)
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Product Name 

GGA3 antibody

 

See all GGA3 antibodies (4)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to GGA3

Immunogen 

Synthetic peptide: ADPAPNVPPKESAGNS conjugated to KLH, corresponding to amino acids 400-415 of Human GGA3.
BLAST 'ADPAPNVPPKESAGNS' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

IHC (PFA fixed), WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
WB: 1/500 - 1/3000. Predicted molecular weight: 78 kDa.
Immunohistochemistry (PFA fixed): 1/40 from (PubMed:17116753).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Golgi Apparatus - trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein.

Research areas 

Signal Transduction >> Protein Trafficking >> Golgi Proteins

Relevance 

This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network (TGN) and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma- adaptins. GGA3 mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Alternative splicing of this gene results in two transcript variants.

 

Alternative names GGA3 antibody (ab10553)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

23163    (Human)

GeneCard

    

GC17M070744    (Human)

Omim

    

606006    (Human)

SwissProt

    

Q9NZ52    (Human)

Unigene

    

87726    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.620 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Expiration date is 1 year from date of opening.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GGA3 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for GGA3 antibody images, references, products related to ab10553 and other tools.

 

GGA3 antibody images:

Product image  Western blot - GGA3 antibody (ab10553)

Western blot - GGA3 antibody (ab10553)

GGA3 antibody (ab10553) at 1/600 dilution + HEK293 lysate from cells transfected with GFP-GGA3 fusion protein at 35 µg

Secondary
IRDye 800 conjugated Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution

Predicted band size : 78 kDa


A 110kDa band (see arrow) corresponding to the GFP-GGA3 fusion protein is detected by ab10553.

 

References for GGA3 antibody (ab10553)

Specific publication references   This product has been used in:

Pak Y et al. Transport of LAPTM5 to lysosomes requires association with the ubiquitin ligase Nedd4, but not LAPTM5 ubiquitination. J Cell Biol 175:631-45 (2006). PubMed: 17116753

If you publish research using ab10553 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to GGA3

 

GGA3 antibody - more information

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