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Anti-GGA3 antibody (ab10553)

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Overview

Product name

Anti-GGA3 antibody
See all GGA3 products (6) ...

Description

Rabbit polyclonal to GGA3

Tested applications

WB, IHC (PFA fixed)more details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide: ADPAPNVPPKESAGNS conjugated to KLH, corresponding to amino acids 400-415 of Human GGA3.

ADPAPNVPPK ESAGNS

General notes

Expiration date is 1 year from date of opening.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - GGA3 antibody (ab10553)Western blot - GGA3 antibody (ab10553) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab10553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.

Tissue specificity

Ubiquitously expressed.

Sequence similarities

Belongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain.

Domain

The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function.

Post-translational
modifications

Phosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
Ubiquitinated.

Cellular localization

Golgi apparatus > trans-Golgi network membrane. Endosome membrane.

Target information above from: UniProt accessionQ9NZ52 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ARF-binding protein 3 antibody
  • gamma ear-containing antibody
  • ADP ribosylation factor binding protein 3 antibody
  • ADP ribosylation factor binding protein GGA 3 antibody
  • ADP ribosylation factor binding protein GGA3 antibody
  • ADP-ribosylation factor binding protein GGA3 antibody
  • ADP-ribosylation factor-binding protein GGA3 antibody
  • ARF binding protein GGA 3 antibody
  • ARF binding protein GGA3 antibody
  • ARF-binding protein 3 antibody
  • Gamma adaptin ear containing antibody
  • Gamma-ear containing antibody
  • GGA 3 antibody
  • GGA3 antibody
  • GGA3_HUMAN antibody
  • Golgi associated antibody
  • Golgi associated gamma adaptin ear containing ARF binding protein 3 antibody
  • Golgi localized gamma ear containing ARF binding protein 3 antibody
  • Golgi-localized antibody
  • KIAA0154 antibody
see all

Anti-GGA3 antibody images:

  Western blot - GGA3 antibody (ab10553)

Western blot - GGA3 antibody (ab10553)

Anti-GGA3 antibody (ab10553) at 1/600 dilution + HEK293 lysate from cells transfected with GFP-GGA3 fusion protein at 35 µg

Secondary
IRDye 800 conjugated Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution

Predicted band size : 78 kDa


A 110kDa band (see arrow) corresponding to the GFP-GGA3 fusion protein is detected by ab10553.

References for Anti-GGA3 antibody (ab10553)

This product has been referenced in:

  • Koike MAet al. Oligemic hypoperfusion differentially affects tau and amyloid-{beta}. Am J Pathol 177:300-10 (2010). WB; Mouse.Read more (PubMed: 20472896) »
  • Pak Yet al. Transport of LAPTM5 to lysosomes requires association with the ubiquitin ligase Nedd4, but not LAPTM5 ubiquitination. J Cell Biol 175:631-45 (2006).Read more (PubMed: 17116753) »

See all 2 publications for this product

Publishing research using ab10553? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"