|
|
|
|
Western blot - GGA3 antibody (ab10553)
(enlarge)
|
|
|
|
|
Product Name
|
GGA3 antibody
|
| |
See all GGA3 antibodies (4)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to GGA3
|
|
Immunogen
|
Synthetic peptide: ADPAPNVPPKESAGNS conjugated to KLH, corresponding to amino acids 400-415 of Human GGA3.
BLAST 'ADPAPNVPPKESAGNS' with  or 
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
IHC (PFA fixed), WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
WB: 1/500 - 1/3000. Predicted molecular weight: 78 kDa.
Immunohistochemistry (PFA fixed): 1/40 from (PubMed:17116753).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Golgi Apparatus - trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein.
|
|
Research areas
|
Signal Transduction >> Protein Trafficking >> Golgi Proteins
|
|
Relevance
|
This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network (TGN) and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma- adaptins. GGA3 mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Alternative splicing of this gene results in two transcript variants.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.620 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
|
Notes
|
Expiration date is 1 year from date of opening.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GGA3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for GGA3 antibody images, references, products related to ab10553 and other tools.
|
| |
GGA3 antibody images:
|
 Western blot - GGA3 antibody (ab10553)
GGA3 antibody (ab10553) at 1/600 dilution + HEK293 lysate from cells transfected with GFP-GGA3 fusion protein at 35 µg
Secondary
IRDye 800 conjugated Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size : 78 kDa
A 110kDa band (see arrow) corresponding to the GFP-GGA3 fusion protein is detected by ab10553.
|
| |
References for GGA3 antibody (ab10553)
|
|
This product has been used in:
Pak Y et al. Transport of LAPTM5 to lysosomes requires association with the ubiquitin ligase Nedd4, but not LAPTM5 ubiquitination. J Cell Biol 175:631-45 (2006). PubMed: 17116753
If you publish research using ab10553 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to GGA3
|
| |
GGA3 antibody - more information
|
Customer reviews (feedback) regarding GGA3 antibody |
Customer FAQs regarding GGA3 antibody |
Protocols for GGA3 antibody |
Price and availability of products related to GGA3 antibody |
| |
GGA3 antibody - related products:
|
|
|
| |
GGA3 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about GGA3 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
