Anti-GGA3 antibody (ab10553)

Overview

• Product nameAnti-GGA3 antibodySee all GGA3 primary antibodies ...
• Description
  Rabbit polyclonal to GGA3
• Tested applicationsWB, IHC (PFA fixed) more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide: ADPAPNVPPKESAGNS conjugated to KLH, corresponding to amino acids 400-415 of Human GGA3.

  Run BLAST with Run BLAST with
• General notesExpiration date is 1 year from date of opening.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Trafficking
  • Golgi Proteins

Applications

Target

• FunctionPlays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
• Tissue specificityUbiquitously expressed.
• Sequence similaritiesBelongs to the GGA protein family.
  Contains 1 GAE domain.
  Contains 1 GAT domain.
  Contains 1 VHS domain.
• DomainThe VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
  The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
  The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.
  The GAE domain binds accessory proteins regulating GGAs function.
• Post-translational
  modificationsPhosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
  Ubiquitinated.
• Cellular localizationGolgi apparatus > trans-Golgi network membrane. Endosome membrane.

Target information above from: UniProt accession Q9NZ52 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 23163 Human
  • Entrez Gene: 23163 Human
  • Entrez Gene: 23163 Human
  • Omim: 606006 Human
  • SwissProt: Q9NZ52 Human
  • SwissProt: Q9NZ52 Human
  • SwissProt: Q9NZ52 Human
  • Unigene: 87726 Human

• Alternative names
  ADP ribosylation factor binding protein 3 antibodyADP ribosylation factor binding protein GGA 3 antibodyADP ribosylation factor binding protein GGA3 antibody

  ADP-ribosylation factor binding protein GGA3 antibodyADP-ribosylation factor-binding protein GGA3 antibodyARF binding protein GGA 3 antibodyARF binding protein GGA3 antibodyARF-binding protein 3 antibodyARF-binding protein 3 antibodyGamma adaptin ear containing antibodygamma ear-containing antibodyGamma-ear containing antibodyGGA 3 antibodyGGA3 antibodyGGA3_HUMAN antibodyGolgi associated antibodyGolgi associated gamma adaptin ear containing ARF binding protein 3 antibodyGolgi localized gamma ear containing ARF binding protein 3 antibodyGolgi-localized antibodyKIAA0154 antibody

  see all

Anti-GGA3 antibody images

• 

  Western blot - GGA3 antibody (ab10553)
  Anti-GGA3 antibody (ab10553) at 1/600 dilution + HEK293 lysate from cells transfected with GFP-GGA3 fusion protein at 35 µg
  
  Secondary
  IRDye 800 conjugated Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
  
  Predicted band size : 78 kDa