Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Protein Trafficking >> Golgi Proteins
Overview
Product name
Anti-GGA3 antibody
See all GGA3 products (6) ...
Description
Rabbit polyclonal to GGA3
Tested applications
WB, IHC (PFA fixed)more details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: ADPAPNVPPKESAGNS conjugated to KLH, corresponding to amino acids 400-415 of Human GGA3.
ADPAPNVPPK ESAGNS
General notes
Expiration date is 1 year from date of opening.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Golgi Proteins
-
Western blot - GGA3 antibody (ab10553)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/3000.Predicted molecular weight: 78 kDa.
IHC (PFA fixed): 1/40(PubMed: 17116753)
Target
Function
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain.
Domain
The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function.
Post-translational
modifications
Phosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
Ubiquitinated.
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
Target information above from: UniProt accessionQ9NZ52
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ARF-binding protein 3 antibody
- gamma ear-containing antibody
- ADP ribosylation factor binding protein 3 antibody
see all
Database links
- Entrez Gene: 23163 Human
- Omim: 606006 Human
- SwissProt: Q9NZ52 Human
- Unigene: 87726 Human
Anti-GGA3 antibody images:
Western blot - GGA3 antibody (ab10553)
Anti-GGA3 antibody (ab10553) at 1/600 dilution + HEK293 lysate from cells transfected with GFP-GGA3 fusion protein at 35 µg
Secondary
IRDye 800 conjugated Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size : 78 kDa
A 110kDa band (see arrow) corresponding to the GFP-GGA3 fusion protein is detected by ab10553.
References for Anti-GGA3 antibody (ab10553)
This product has been referenced in:
- Koike MAet al. Oligemic hypoperfusion differentially affects tau and amyloid-{beta}. Am J Pathol 177:300-10 (2010). WB; Mouse.Read more (PubMed: 20472896) »
- Pak Yet al. Transport of LAPTM5 to lysosomes requires association with the ubiquitin ligase Nedd4, but not LAPTM5 ubiquitination. J Cell Biol 175:631-45 (2006).Read more (PubMed: 17116753) »
See all 2 publications for this product
Publishing research using ab10553? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"