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Anti-GGA3 antibody (ab76069)

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Overview

Product name

Anti-GGA3 antibody
See all GGA3 products (6) ...

Description

Rabbit polyclonal to GGA3

Tested applications

WB, IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Rat, Human

Predicted to work with

Mouse, Chicken

Immunogen

A synthetic peptide from the VHS domain of human GGA3 conjugated to an immunogenic carrier protein was used as the immunogen.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Whole serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

  • Western blot - GGA3 antibody (ab76069)Western blot - GGA3 antibody (ab76069) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab76069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/300 - 1/2000.Predi...Read more →

    WB: 1/300 - 1/2000.Predicted molecular weight: 78 kDa.

  • ShowHide

    IHC-P

     IHC-P: 1/300 - 1/2000.(A...Read more →

    IHC-P: 1/300 - 1/2000.(Antigen retrieval step is recommended.)

  • IHC-Fr

     IHC-Fr: 1/300 - 1/2000.

    IHC-Fr: 1/300 - 1/2000.

Target

Function

Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.

Tissue specificity

Ubiquitously expressed.

Sequence similarities

Belongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain.

Domain

The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function.

Post-translational
modifications

Phosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
Ubiquitinated.

Cellular localization

Golgi apparatus > trans-Golgi network membrane. Endosome membrane.

Target information above from: UniProt accessionQ9NZ52 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ARF-binding protein 3 antibody
  • gamma ear-containing antibody
  • ADP ribosylation factor binding protein 3 antibody
  • ADP ribosylation factor binding protein GGA 3 antibody
  • ADP ribosylation factor binding protein GGA3 antibody
  • ADP-ribosylation factor-binding protein GGA3 antibody
  • ARF binding protein GGA 3 antibody
  • ARF binding protein GGA3 antibody
  • GGA 3 antibody
  • GGA3 antibody
  • GGA3_HUMAN antibody
  • Golgi associated gamma adaptin ear containing ARF binding protein 3 antibody
  • Golgi localized gamma ear containing ARF binding protein 3 antibody
  • Golgi-localized antibody
  • KIAA0154 antibody
see all

Anti-GGA3 antibody images:

  Western blot - GGA3 antibody (ab76069)

Western blot - GGA3 antibody (ab76069)

All lanes : Anti-GGA3 antibody (ab76069) at 1/100 dilution

Lane 1 : Rat brain lysates
Lane 2 : Rat brain lysates treated with ab76069 pre-absorbed with Immunizing peptide


Performed under reducing conditions.

Predicted band size : 78 kDa

References for Anti-GGA3 antibody (ab76069)

ab76069 has not yet been referenced specifically in any publications.

Publishing research using ab76069? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"