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Products:Signal Transduction >> Protein Trafficking >> Golgi Proteins
Overview
Product name
Anti-GGA3 antibody
See all GGA3 products (6) ...
Description
Rabbit polyclonal to GGA3
Tested applications
WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse, Chicken
Immunogen
A synthetic peptide from the VHS domain of human GGA3 conjugated to an immunogenic carrier protein was used as the immunogen.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Golgi Proteins
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Western blot - GGA3 antibody (ab76069)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/300 - 1/2000.Predicted molecular weight: 78 kDa.
IHC-P: 1/300 - 1/2000.(Antigen retrieval step is recommended.)
IHC-Fr
IHC-Fr: 1/300 - 1/2000.IHC-Fr: 1/300 - 1/2000.
Target
Function
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain.
Domain
The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function.
Post-translational
modifications
Phosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
Ubiquitinated.
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
Target information above from: UniProt accessionQ9NZ52
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ARF-binding protein 3 antibody
- gamma ear-containing antibody
- ADP ribosylation factor binding protein 3 antibody
see all
Database links
- Entrez Gene: 23163 Human
- Entrez Gene: 260302 Mouse
- Entrez Gene: 360658 Rat
- Omim: 606006 Human
- SwissProt: Q9NZ52 Human
- SwissProt: Q8BMI3 Mouse
- Unigene: 87726 Human
- Unigene: 119479 Mouse
see all
Anti-GGA3 antibody images:
Western blot - GGA3 antibody (ab76069)
All lanes : Anti-GGA3 antibody (ab76069) at 1/100 dilution
Lane 1 : Rat brain lysates
Lane 2 : Rat brain lysates treated with ab76069 pre-absorbed with Immunizing peptide
Performed under reducing conditions.
Predicted band size : 78 kDa
References for Anti-GGA3 antibody (ab76069)
ab76069 has not yet been referenced specifically in any publications.
Publishing research using ab76069? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"