Anti-GGT1 antibody (ab96466)

Overview

• Product nameAnti-GGT1 antibodySee all GGT1 primary antibodies ...
• Description
  Rabbit polyclonal to GGT1
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Pig
• Immunogen

  Recombinant fragment containing a sequence corresponding to a region within amino acids 1-196 of Human GGT1 (NP_005256).

• Positive controlMOLT4 whole cell lysate; 293T, A431, H1299, HeLaS3, HepG2 and Raji cell lysates

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
  Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Amino acid metabolism

  • Signal Transduction
  • Metabolism
  • Amino Acids

Applications

Target

• FunctionInitiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracelular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive.
• Tissue specificityDetected in fetal and adult kidney and liver, adult pancreas, stomach, intestine, placenta and lung. Isoform 3 is lung-specific. There are several other tissue-specific forms that arise from alternative promoter usage but that produce the same protein.
• PathwaySulfur metabolism; glutathione metabolism.
• Involvement in diseaseDefects in GGT1 are a cause of glutathionuria (GLUTH) [MIM:231950]; also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease.
• Sequence similaritiesBelongs to the gamma-glutamyltransferase family.
• Post-translational
  modificationsN-glycosylated on both chains. Contains hexoses, hexosamines and sialic acid residues. Glycosylation profiles tested in kidney and liver tissues reveal the presence of tissue-specific and site-specific glycan composition, despite the overlap in composition among the N-glycans. A total of 36 glycan compositions, with 40 unique structures are observed. Up to 15 different glycans are observed at a single site, with site-specific variation in glycan composition. The difference in glycosylation profiles in the 2 tissues do not affect the enzyme activity.
• Cellular localizationMembrane.

Target information above from: UniProt accession P19440 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2678 Human
  • Omim: 612346 Human
  • SwissProt: P19440 Human
  • SwissProt: P20735 Pig
  • Unigene: 595809 Human
  • Unigene: 645535 Human

• Alternative names
  CD224 antibodyD22S672 antibodyD22S732 antibody

  Gamma glutamyl transpeptidase antibodyGamma glutamyltransferase 1 antibodyGamma glutamyltranspeptidase 1 antibodyGamma-glutamyltransferase 1 antibodyGamma-glutamyltranspeptidase 1 light chain antibodyGGT 1 antibodyGGT antibodyGGT1 antibodyGGT1_HUMAN antibodyGlutamyl transpeptidase antibodyGTG antibodyMGC96892 antibodyMGC96904 antibodyMGC96963 antibodyOTTHUMP00000028921 antibodyOTTHUMP00000197959 antibody

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Anti-GGT1 antibody images

• 

  Western blot - GGT1 antibody (ab96466)
  Anti-GGT1 antibody (ab96466) at 1/500 dilution + MOLT4 whole cell lysate at 30 µg
  
  Predicted band size : 61 kDa