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Anti-GHDC antibody (ab69269)

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Overview

Product name

Anti-GHDC antibody
See all GHDC products (3) ...

Description

Mouse polyclonal to GHDC

Specificity

This antibody is reactive against mammalian transfected lysate.

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Full length protein, corresponding to amino acids 1-530 of Human GHDC

Positive control

Transfected 293T cell lysate and human kidney tissue lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 1X PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - GHDC antibody (ab69269)Western blot - GHDC antibody (ab69269) image (enlarge)

  • Western blot - GHDC antibody (ab69269)Western blot - GHDC antibody (ab69269) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab69269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).
Detection Antibody.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Sequence similarities

Belongs to the GH3 family.

Cellular localization

Endoplasmic reticulum. Nucleus envelope.

Target information above from: UniProt accessionQ8N2G8 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • D11LGP1 antibody
  • D11LGP1E antibody
  • D11lgp1e like antibody
  • GH3 domain containing antibody
  • GH3 domain containing protein antibody
  • GH3 domain-containing protein antibody
  • GHDC antibody
  • GHDC_HUMAN antibody
  • Homolog of mouse LGP1 antibody
  • LGP1 antibody
see all

Anti-GHDC antibody images:

  Western blot - GHDC antibody (ab69269)

Western blot - GHDC antibody (ab69269)

Anti-GHDC antibody (ab69269) at 1/500 dilution + human kidney tissue lysate at 25 µg

Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size : 58 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)

  Western blot - GHDC antibody (ab69269)

Western blot - GHDC antibody (ab69269)

All lanes : Anti-GHDC antibody (ab69269) at 1/500 dilution

Lane 1 : GHDC transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate

Lysates/proteins at 25 µg per lane.

Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size : 58 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-GHDC antibody (ab69269)

ab69269 has not yet been referenced specifically in any publications.

Publishing research using ab69269? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"