Anti-GIGYF1 antibody (ab177509)
Key features and details
- Rabbit polyclonal to GIGYF1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-GIGYF1 antibody
See all GIGYF1 primary antibodies -
Description
Rabbit polyclonal to GIGYF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Human GIGYF1 aa 350-400. The exact sequence is proprietary. (NP_072096.2)
Sequence:E AGGKELTPLP PQEEKSSSPS PLPTLGPLWG TNGDGDETAE KEPPAAEDDI
Database link: O75420 -
Positive control
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 - 8. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab177509 was affinity purified using an epitope specific to GIGYF1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 115 kDa.
|
|
IP |
Use at 2-10 µg/mg of lysate.
|
Notes |
---|
WB
1/1000 - 1/5000. Predicted molecular weight: 115 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
-
Function
May act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. May increase IGF1 receptor phosphorylation under IGF1 stimulation as well as phosphorylation of IRS1 and SHC1. -
Sequence similarities
Belongs to the PERQ family.
Contains 1 GYF domain. -
Domain
The GYF domain interacts with GRB10. - Information by UniProt
-
Database links
- Entrez Gene: 472671 Chimpanzee
- Entrez Gene: 532406 Cow
- Entrez Gene: 101139578 Gorilla
- Entrez Gene: 64599 Human
- Omim: 612064 Human
- SwissProt: O75420 Human
- Unigene: 414396 Human
-
Alternative names
- CDS2 antibody
- GIGYF1 antibody
- GRB10-interacting GYF protein 1 antibody
see all
Images
-
All lanes : Anti-GIGYF1 antibody (ab177509) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 115 kDa
Exposure time: 10 seconds -
Detection of GIGYF1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab177509 at 6 µg/mg lysate for IP (Lane 1). For WB detection, ab177509 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab177509 has not yet been referenced specifically in any publications.