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Anti-GIP antibody [04] (ab30679)

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Overview

Product name

Anti-GIP antibody [04]
See all GIP products (4) ...

Description

Mouse monoclonal [04] to GIP

Specificity

Ab30679 recognises GIP. It cross reacts up to 10% with PACAP and GLP2 but <0.1% with glucagon, GLP1 or VIP.

Tested applications

IHC-P, ELISAmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic human GIP coupled to carrier. The immunogen was adsorbed onto Al(OH)3 and emulsified in Freund's incomplete adjuvant.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide.
Constituents: 500mM Sodium chloride, 10mM phosphate buffer. pH 7.4.

Concentration

Concentration information loading...

Purity

Protein A purified

Primary antibody notes

Fusion partner: SP2/mIL6.

Clonality

Monoclonal

Clone number

04

Isotype

IgG1

Light chain type

kappa

Research areas

Signal Transduction >> Growth Factors/Hormones >> Hormones
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GIP antibody [04] (ab30679)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GIP antibody [04] (ab30679) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GIP antibody [04] (ab30679)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GIP antibody [04] (ab30679) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab30679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: 1/10000. This dilution guideline applies to glutaraldehyde linked GIP OA (molar ratio 4:1) coated at 1 mg/mL. With GIP coated directly at 2 µg/mL the dilution guideline is 1/2000.
IHC-P: 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6.0 before commencing with IHC staining protocol.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Gastric inhibitory peptide, GIP, is a 42 amino acid residue protein and is principally produced in K cells in the duodenum, jejunum and, occasionally, upper ileum. GIP may also be expressed in non intestinal tissues such as rat salivary glands. It is a potent stimulator of insulin secretion in hyperglycemia. GIP acts by binding to specific G protein coupled receptors on the pancreatic islet cell membrane. An internal domain of GIP may be responsible and that the mechanism of ligand activated signal transduction is via a wortmannin sensitive pathway.

Cellular localization

Secreted

Alternative names

  • Gastric Inhibitory Peptide antibody
  • Gastric inhibitory polypeptide antibody
  • Gastric inhibitory polypeptide precursor antibody
  • GIP antibody
  • Glucose dependent insulinotropic polypeptide antibody
  • Glucose-dependent insulinotropic polypeptide antibody
see all

Database links

Anti-GIP antibody [04] images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GIP antibody [04] (ab30679)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GIP antibody [04] (ab30679)

ab30679 at 1/100 staining GIP in human duodenum by IHC-P.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GIP antibody [04] (ab30679)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GIP antibody [04] (ab30679)

ab30679 at 1/100 staining GIP in human small intestine by IHC-P.

References for Anti-GIP antibody [04] (ab30679)

ab30679 has not yet been referenced specifically in any publications.

Publishing research using ab30679? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"