GIP antibody (ab22624)

Product Name 

GIP antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to GIP

Immunogen 

Full length native protein (purified) (Pig) conjugated to KLH by a CDI linker.

Reacts with 
(species key)

Pig

Specificity 

ab22624 reacts with Glucose-dependent Insulinotropic Peptide (GIP).

Tested applications 
(see key)

IHC-P, IHC-R

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IHC-P: 1/1000 - 1/8000 (using method A) and 1/200-1/1200 (using method B)
IHC-R: 1/1000 - 1/8000 (using method A) and 1/200-1/1200 (using method B)
Notes:
Dilute to working strength with phosphatebuffered saline (PBS) containing 0.1% w/v sodium azide.
Method A: using overnight incubation and indirect immunoperoxidase
procedures, such as PAP and ABCperoxidase.
Method B: using overnight incubation and indirect immunofluorescence procedures.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mammalian duodenal (K) endocrine cells.

Cellular localization 

Secreted

Research areas 

Signal Transduction >> Growth Factors/Hormones >> Hormones
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR

Relevance 

Gastric inhibitory peptide, GIP, is a 42 amino acid residue protein and is principally produced in K cells in the duodenum, jejunum and, occasionally, upper ileum. GIP may also be expressed in non intestinal tissues such as rat salivary glands. It is a potent stimulator of insulin secretion in hyperglycemia. GIP acts by binding to specific G protein coupled receptors on the pancreatic islet cell membrane. An internal domain of GIP may be responsible and that the mechanism of ligand activated signal transduction is via a wortmannin sensitive pathway.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: Whole serum

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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