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Product name
Anti-GIPC1 antibody
See all GIPC1 products (5) ...
Description
Goat polyclonal to GIPC1
Tested applications
IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide: GAIGDAKVGRY, corresponding to C terminal amino acids 323-333 of Human GIPC1.
C-GAIGDAKVGRY
Positive control
HeLa Lysate.
General notes
GenBank Accession Number – NP_005707
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 10 µg/ml. In paraffin embedded Human Kidney, shows membrane and/or cytoplasmic staining of epithelial cells of renal tubules and some rare cells of the glomeruli.
WB: Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
ICC/IF: Use at an assay dependent dilution. PubMed: 20634288
Target
Relevance
GIPC1 is a hydrophilic protein that contains 333 amino acids, including multiple phosphorylation sites and an 80 to 100 amino acid PDZ domain. PDZ domain-containing proteins typically recognize C terminal amino acids and are involved in protein network signaling. GIPC1 may be involved in G protein-linked signaling.
Cellular localization
Cell Membrane, peripheral membrane protein and Cytoplasmic.
Alternative names
- C19ORF3 antibody
- Chromosome 19 open reading frame 3 antibody
- GIPC antibody
see all
Database links
- Entrez Gene: 10755 Human
- Entrez Gene: 67903 Mouse
- Entrez Gene: 83823 Rat
- NCBI: NP_005707.1 Human
- Omim: 605072 Human
- SwissProt: O14908 Human
- SwissProt: Q9Z0G0 Mouse
- SwissProt: Q9Z254 Rat
see all
Anti-GIPC1 antibody images:
Western blot - GIPC1 antibody (ab5951)
Predicted band size : 38 kDa
ab5951 at 1µg/ml staining GIPC1 at approximately 40kDa in HeLa Lysate by Western blot (ECL).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GIPC1 antibody (ab5951)
Ab5951 (10ug/ml) staining human GIPC1 in human kidney by immunohistochemistry using paraffin embedded tissue.
Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Western blot - GIPC1 antibody (ab5951)
Anti-GIPC1 antibody (ab5951) at 0.03 µg/ml + Human HeLa cell lysate (RIPA buffer, 35µg total protein per lane)
Predicted band size : 38 kDa
Observed band size : 24,38 kDa (why is the actual band size different from the predicted?)
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GIPC1 antibody (ab5951)
ab5951 staining GIPC1 in human prostate tissue section by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formalin fixed prior to blocking in 10% serum for 20 minutes at RT. The primary antibody was diluted 1/250 and incubated with the sample for 24 hour at 4°C. A biotin conjugated rabbit anti-goat antibody, diluted 1/250, was used as the secondary.
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence-GIPC1 antibody(ab5951)
ICC/IF image of ab5951 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5951, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-GIPC1 antibody (ab5951)
This product has been referenced in:
- Wu D et al. GIPC1 interacts with MyoGEF and promotes MDA-MB-231 breast cancer cell invasion. J Biol Chem 285:28643-50 (2010). ICC/IF; Human.Read more (PubMed: 20634288) »
- Valdembri D et al. Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic and function in endothelial cells. PLoS Biol 7:e25 (2009). WB; Human.Read more (PubMed: 19175293) »
See all 3 publications for this product
Publishing research using ab5951? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"