Recombinant Anti-Cx30 antibody [16H9L8] (ab200866)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [16H9L8] to Cx30
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Cx30 antibody [16H9L8]
See all Cx30 primary antibodies -
Description
Rabbit monoclonal [16H9L8] to Cx30 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide corresponding to Mouse Cx30 aa 200 to the C-terminus.
Database link: P70689 -
Positive control
- WB: Adult mouse thalamus lysates. IF: Log phase Caco-2 cells. IHC: Adult mouse thalamus tissue .
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
16H9L8 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 0.5 - 1 µg/ml.
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WB |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 30 kDa.
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ICC/IF |
Use a concentration of 0.5 - 1 µg/ml.
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Notes |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. |
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 30 kDa. |
ICC/IF
Use a concentration of 0.5 - 1 µg/ml. |
Target
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Function
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. -
Involvement in disease
Defects in GJB6 are the cause of ectodermal dysplasia type 2 (ED2) [MIM:129500]; also known as Clouston syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ED2 is an autosomal dominant condition characterized by atrichosis, nail hypoplasia and deformities, hyperpigmentation of the skin, normal teeth, normal sweat and sebaceous gland function. Palmoplantar hyperkeratosis is a frequent features. Hearing impairment has been detected in few cases of ED2.
Defects in GJB6 are the cause of deafness autosomal recessive type 1B (DFNB1B) [MIM:612645]. DFNB1B is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Defects in GJB6 are the cause of deafness autosomal dominant type 3B (DFNA3B) [MIM:612643]. -
Sequence similarities
Belongs to the connexin family. Beta-type (group I) subfamily. -
Cellular localization
Cell membrane. Cell junction > gap junction. - Information by UniProt
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Database links
- Entrez Gene: 10804 Human
- Entrez Gene: 14623 Mouse
- Omim: 604418 Human
- SwissProt: O95452 Human
- SwissProt: P70689 Mouse
- Unigene: 511757 Human
- Unigene: 25652 Mouse
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Alternative names
- Connexin 30 antibody
- Connexin-30 antibody
- Cx30 antibody
see all
Images
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Immunohistochemical analysis of adult mouse thalamus tissue labeling Cx30 with ab200866 at 0.5 µg/ml.
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Anti-Cx30 antibody [16H9L8] (ab200866) at 1 µg/ml + Adult mouse thalamus lysate
Predicted band size: 30 kDa -
Immunofluorescence analysis of 4% paraformaldehyde-fixed log phase Caco-2 cells labeling Cx30 with ab200866 at 1/500 dilution. Alexa Fluor® 488 Goat Anti-Rabbit IgG Secondary Antibody at a dilution of 1/400 was used as the secondary antibody (panel a; green). Slide is counter stained with Dapi (panel b: blue). Panel c is a merged image showing cell junction localization and panel d is a no primary antibody control. The images were captured using a Nikon microscope at 20X magnification.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab200866 has been referenced in 1 publication.
- Dang R et al. Edaravone ameliorates depressive and anxiety-like behaviors via Sirt1/Nrf2/HO-1/Gpx4 pathway. J Neuroinflammation 19:41 (2022). PubMed: 35130906