Anti-GLT25D1 antibody (ab151011)
Key features and details
- Rabbit polyclonal to GLT25D1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GLT25D1 antibody -
Description
Rabbit polyclonal to GLT25D1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig -
Immunogen
Recombinant fragment corresponding to amino acids 269-336 of Human GLT25D1.
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Positive control
- U-251 MG cell lysate; Human placenta tissue
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General notes
The antibody solution should be gently mixed before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 72 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 72 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Cellular localization
Endoplasmic reticulum lumen. Note: Colocalized with PLOD3 and mannose binding lectin. -
Database links
- Entrez Gene: 513167 Cow
- Entrez Gene: 484834 Dog
- Entrez Gene: 100070236 Horse
- Entrez Gene: 79709 Human
- Entrez Gene: 234407 Mouse
- Entrez Gene: 290637 Rat
- SwissProt: A5PK45 Cow
- SwissProt: Q8NBJ5 Human
see all -
Alternative names
- FLJ22329 antibody
- Glycosyltransferase 25 domain containing 1 antibody
- Hydroxylysine galactosyltransferase 1 antibody
- Procollagen galactosyltransferase 1 antibody
Images
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All lanes : Anti-GLT25D1 antibody (ab151011) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Predicted band size: 72 kDa -
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling GLT25D1 with ab151011 at 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151011 has been referenced in 1 publication.
- Baumann S & Hennet T Collagen Accumulation in Osteosarcoma Cells lacking GLT25D1 Collagen Galactosyltransferase. J Biol Chem 291:18514-24 (2016). WB . PubMed: 27402836