GM-CSF Rat ELISA Kit (ab100762)
- Product nameGM-CSF Rat ELISA KitSee all GM-CSF kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 10 pg/ml
- Range12.35 pg/ml - 3000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 90.53 81% - 101% Serum 136.4 128% - 143% Plasma 118.8 88% - 136%
- Species reactivityReacts with: Rat
- Product overview
ab100762 Rat GM-CSF (Granulocyte Macrophage Colony Stimulating Factor) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat GM-CSF in serum, plasma and cell culture supernatants. This assay employs an antibody specific for GM-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and GM-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat GM-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 400X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent B 5x concentrated buffer. ForStandard/Sample (cell culture medium) diluent. 1 x 15ml Assay Diluent C diluent buffer . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Detection Antibody GM-CSF: biotinylated anti-rat GM-CSF 2 vials GM-CSF Microplate (12 strips x 8 wells) coated with anti-rat GM-CSF 1 unit GM-CSF Standard Rat (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- Colony stimulating factor Colony stimulating factor 2 (granulocyte macrophage) CSF
- CSF 2CSF2GMCSF Granulocyte macrophage colony stimulating factorMGC131935MGC138897Molgramostin Sargramostim
Our Abpromise guarantee covers the use of ab100762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for GM-CSF Rat ELISA Kit (ab100762)
ab100762 has not yet been referenced specifically in any publications.