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GM-CSF Receptor alpha protein (Active) (ab83993)

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Overview

  • Product nameGM-CSF Receptor alpha protein (Active)See all GM-CSF Receptor alpha proteins and peptides ...
  • Protein descriptionRecombinant full length extracellular domain (amino acids 23-320) of human GM-CSF Receptor alpha, fused to the Fc region of human IgG1 (aa 90-330) expressed in modified human 293 cells (Swiss-Prot P15509).
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activityab83993 bound to protein A sepharose beads was able to pull down its ligand, GM-CSF.
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsStore at +4°C.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin
  • Concentration information loading...
  • Sequence notesTheoretical Sequence: EKSDLRTVAPASSLNVRFDSRTMNLSWDCQENTTFSKC FLTDKKNRVV EPRLSNNECSCTFREICLHEGVTFEVHV NTSQRGFQQKLLYPNSGREG TAAQNFSCFIYNADLMNC TWARGPTAPRDVQYFLYIRNSKRRREIRCP YYIQDSGT HVGCHLDNLSGLTSRNYFLVNGTSREIGIQFFDSLLDTKK IERFNPPSNVTVRCNTTHCLVRWKQPRTYQKLSYLDFQ YQLDVHRKNT QPGTENLLINVSGDLENRYNFPSSEPRA KHSVKIRAADVRILNWSSWS EAIEFGSDDGGSSNTKVD KKVEPKSCDKTHTCPPCPAPELLGGPSVFL FPPKPKDT LMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTK PREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKAL PAPIEKTISK AKGQPREPQVYTLPPSRDELTKNQVSLT CLVKGFYPSDIAVEWESNGQ PENNYKTTPPVLDSDGSF FLYSKLTVDKSRWQQGNVFSCSVMHEALHN HYTQKSLS LSPGK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab83993 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    Molecular Mass: Under reducing conditions ab83993 migrates as a broad band between 75 and 100 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified GM-CSF Receptor alpha (Fc Chimera) that has a predicted monomeric molecular mass of 61.7 kDa. ab83993 consists of 15-40% carbohydrate by weight.

    pI: ab83993 separates into a number of isoforms with a pI between 6.0 and 9.5 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified GM-CSF Receptor alpha (Fc Chimera) that has a predicted pI of 8.7.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        CD116CD116 antigenCDw116
        Colony stimulating factor 2 receptor alpha chainColony stimulating factor 2 receptor alpha low affinityCSF 2RCSF2RCSF2R_HUMANCSF2RACSF2RAXCSF2RAYCSF2RXCSF2RYGM CSF R alphaGM CSF receptor alpha subunitGM-CSF-R-alphaGMCSFRGMCSFR-alphaGMR-alphaGranulocyte-macrophage colony-stimulating factor receptor subunit alphaSMDP4
      see all
  • FunctionLow affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.
  • Involvement in diseaseDefects in CSF2RA are the cause of pulmonary surfactant metabolism dysfunction type 4 (SMDP4) [MIM:300770]. A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 5 subfamily.
  • DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localizationSecreted and Cell membrane.

    • Target information above from: UniProt accession P15509 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    GM-CSF Receptor alpha protein (Active) images

    • Functional Studies - GM-CSF Receptor alpha protein (Fc Chimera Active) (ab83993)
      Functional Studies - GM-CSF Receptor alpha protein (Fc Chimera Active) (ab83993)
      Densitometry of protein isoforms visualised by 2-DE.
      The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
      The triangle indicates the theoretical MW and pI of the protein.
    • SDS-PAGE - GM-CSF Receptor alpha protein (Fc Chimera Active) (ab83993)
      SDS-PAGE - GM-CSF Receptor alpha protein (Fc Chimera Active) (ab83993)
      1D SDS-PAGE of ab83993 before and after treatment with glycosidases to remove oligosaccharides.
      Lane 1: ab83993
      Lane 2: ab83993 treated with PNGase F to remove potential N-linked glycans
      Lane 3: ab83993 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
      Approximately 5 µg of protein was loaded per lane; Gel was stained using Deep Purple™.

      Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A tightening of the band after treatment with the glycosidase cocktail indicates O-linked glycans may be present. Additional bands in lane 2 and lane 3 are glycosidase enzymes.

    References for GM-CSF Receptor alpha protein (Active) (ab83993)

    ab83993 has not yet been referenced specifically in any publications.

    Publishing research using ab83993 ? Please let us know so that we can cite the reference in this datasheet

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