GM-CSF Receptor alpha protein (Active) (ab83993)
Constituents: 10% Trehalose, 1% Human serum albumin
Our Abpromise guarantee covers the use of ab83993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Molecular Mass: Under reducing conditions ab83993 migrates as a broad band between 75 and 100 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified GM-CSF Receptor alpha (Fc Chimera) that has a predicted monomeric molecular mass of 61.7 kDa. ab83993 consists of 15-40% carbohydrate by weight.
pI: ab83993 separates into a number of isoforms with a pI between 6.0 and 9.5 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified GM-CSF Receptor alpha (Fc Chimera) that has a predicted pI of 8.7.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- CD116CD116 antigenCDw116
- Colony stimulating factor 2 receptor alpha chainColony stimulating factor 2 receptor alpha low affinityCSF 2RCSF2RCSF2R_HUMANCSF2RACSF2RAXCSF2RAYCSF2RXCSF2RYGM CSF R alphaGM CSF receptor alpha subunitGM-CSF-R-alphaGMCSFRGMCSFR-alphaGMR-alphaGranulocyte-macrophage colony-stimulating factor receptor subunit alphaSMDP4
The box 1 motif is required for JAK interaction and/or activation.
GM-CSF Receptor alpha protein (Active) images
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MW and pI of the protein.
1D SDS-PAGE of ab83993 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab83993
Lane 2: ab83993 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab83993 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
Approximately 5 µg of protein was loaded per lane; Gel was stained using Deep Purple™. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A tightening of the band after treatment with the glycosidase cocktail indicates O-linked glycans may be present. Additional bands in lane 2 and lane 3 are glycosidase enzymes.
References for GM-CSF Receptor alpha protein (Active) (ab83993)
ab83993 has not yet been referenced specifically in any publications.