Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesab54429 is 0.2µm sterile filtered.
  • Clonality Monoclonal
  • Clone number30-4
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab54429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 17 kDa.
ELISA 1/1000 - 1/2000.
Functional Studies Use at an assay dependent dilution.
ICC/IF Use a concentration of 25 µg/ml.
  • Application notesIs unsuitable for IHC-Fr.
  • Target

    • FunctionCytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
    • Sequence similaritiesBelongs to the GM-CSF family.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Burst Promoting Activity antibody
      • CMCSF antibody
      • Colony stimulating factor 2 (granulocyte-macrophage) antibody
      • Colony Stimulating Factor 2 antibody
      • Colony stimulating factor antibody
      • Colony-stimulating factor antibody
      • CSF 2 antibody
      • CSF Alpha antibody
      • CSF antibody
      • CSF2 antibody
      • CSF2_HUMAN antibody
      • Eosinophil Colony Stimulating Factor antibody
      • GM-CSF antibody
      • GMCSF antibody
      • Granulocyte Macrophage Colony Stimulating Factor antibody
      • Granulocyte-macrophage colony-stimulating factor antibody
      • MGC131935 antibody
      • MGC138897 antibody
      • Molgramostin antibody
      • Pluripoietin Alpha antibody
      • Sargramostim antibody
      see all

    Anti-GM-CSF antibody [30-4] images

    • PBMCs fixed with 4% formaldehyde for 20 minutes at room temperature by adding formaldehyde directly to the culture media and adjust to approximately 1 x 106 cells/mL. Cells pelleted, resuspended and 5 ul (5 x 103) cells added to a gelatin coated slide. Liquid evaporated over a low heat and stained using ab54429 at 10 ug/ml.

    References for Anti-GM-CSF antibody [30-4] (ab54429)

    This product has been referenced in:
    • Isik G  et al. Chimeric HIV-1 envelope glycoproteins with potent intrinsic granulocyte-macrophage colony-stimulating factor (GM-CSF) activity. PLoS One 8:e60126 (2013). Human . Read more (PubMed: 23565193) »
    • van Montfort T  et al. A Chimeric HIV-1 Envelope Glycoprotein Trimer with an Embedded Granulocyte-Macrophage Colony-stimulating Factor (GM-CSF) Domain Induces Enhanced Antibody and T Cell Responses. J Biol Chem 286:22250-61 (2011). Neutralising ; Human . Read more (PubMed: 21515681) »

    See all 2 Publications for this product

    Product Wall

    Comme convenu, J'ai mis en place l'envoi de :

    une unité d'anti-GM CSFab77768 (http://www.abcam.com/ab77768) en remplacement du ab54429 commandé le 15 février 2012, le numéro de commande de ce produit de remplacemen...

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    Merci de nous avoir contactés.

    Le laboratoire m'a envoyé l'image obtenue avec le ab54429 (10µg/ml) en ICC sur cellules mononucléées du sang périphérique (PBMC) fixées en PFA.

    Le...

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    Thank you very much for your interest in ab54429. To our knowledge, ab54429 has not been tested in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab54429 now, test it in IHC-P and submit feedback to us in the form of an Abrev...

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    According to our records, ab54429, ab25123, ab1420 and ab8098 was proving difficult to use and we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting more details in order to help us be...

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    Thank you for contacting us. This antibody has been raised against recombinant human GM-CSF produced in E. coli. I hoe this information has been helpful. If you have any further questions, please do not hesitate to ask.

    Thank you for confirming the details. I would need the purchase order number to send you the requested antibodies. I am sorry without correct purchase order number of each antibody; I will not be able to send you these products. I hope th...

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    Thank you for your enquiry regarding ab1420, ab8098, ab54429 and ab25123 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Additionally, thank you for sup...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"