Directly conjugated antibodies for immunofluorescence

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Overview

Properties

  • FormLyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesab84259 is sterile filtered.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab84259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.25 - 1 µg/ml.

Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human GM-CSF.

Sandwich ELISA Use a concentration of 0.25 - 1 µg/ml.

Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human GM-CSF. Can be paired for Sandwich ELISA with ab9667.  Can be used as detection antibody when paired with recommended pair.

WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 17 kDa.

The detection limit for recombinant human GM-CSF is 1.5 - 3.0 ng/lane under either reducing or non-reducing conditions.

Target

  • FunctionCytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • Sequence similaritiesBelongs to the GM-CSF family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Colony stimulating factor 2 (granulocyte-macrophage) antibody
    • Colony Stimulating Factor 2 antibody
    • Colony stimulating factor antibody
    • Colony-stimulating factor antibody
    • CSF 2 antibody
    • CSF antibody
    • CSF2 antibody
    • CSF2_HUMAN antibody
    • GM-CSF antibody
    • GMCSF antibody
    • Granulocyte Macrophage Colony Stimulating Factor antibody
    • Granulocyte-macrophage colony-stimulating factor antibody
    • MGC131935 antibody
    • MGC138897 antibody
    • MGI1GM antibody
    • Molgramostin antibody
    • Pluripoietin-a antibody
    • Sargramostim antibody
    see all

References for Anti-GM-CSF antibody (Biotin) (ab84259)

ab84259 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"