Overview

Properties

  • FormLyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesab84259 is sterile filtered.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab84259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA
Sandwich ELISA
WB
  • Application notesELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human GM-CSF.
    sELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human GM-CSF.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant human GM-CSF is 1.5 - 3.0 ng/lane under either reducing or non-reducing conditions. Predicted molecular weight: 17 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
    • Sequence similaritiesBelongs to the GM-CSF family.
    • Cellular localizationSecreted.
    • Target information above from: UniProt accession P04141 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • Colony stimulating factor 2 (granulocyte-macrophage) antibody
      • Colony Stimulating Factor 2 antibody
      • Colony stimulating factor antibody
      • Colony-stimulating factor antibody
      • CSF 2 antibody
      • CSF antibody
      • CSF2 antibody
      • CSF2_HUMAN antibody
      • GM-CSF antibody
      • GMCSF antibody
      • Granulocyte Macrophage Colony Stimulating Factor antibody
      • Granulocyte-macrophage colony-stimulating factor antibody
      • MGC131935 antibody
      • MGC138897 antibody
      • Molgramostin antibody
      • Sargramostim antibody
      see all

    References for Anti-GM-CSF antibody (Biotin) (ab84259)

    ab84259 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"