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Overview

  • Product nameAnti-GM-CSF antibody [MP1-31G6]See all GM-CSF primary antibodies ...
  • Description
    Rat monoclonal [MP1-31G6] to GM-CSF
  • Tested applicationsIP, ELISA, Measuring secreted proteins from cells, WB, IHC-P more details
  • Species reactivity
    Reacts with: Mouse

    Does not react with

    Rat
  • Immunogen

    Recombinant full length protein (Mouse) expressed in yeast.

  • General notesAb13789 (for capturing) can be used for Sandwich ELISA with clone MP1-31G6 ab13788, ab45453, ab45470 (for detection).

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is affinity purified.
  • Clonality Monoclonal
  • Clone numberMP1-31G6
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab13789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP
ELISA
ELISPOT
WB
IHC-P
  • Application notesELISA: Use at an assay dependent dilution.
    ELISPOT: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml, this concentration of antibody can detect less than 100 ng of recombinant mouse GM-CSF under reducing conditions. Predicted molecular weight: 17.2 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Alternative names
        Burst Promoting Activity antibodyCMCSF antibodyColony stimulating factor 2 (granulocyte-macrophage) antibody
        Colony Stimulating Factor 2 antibodyColony stimulating factor antibodyColony-stimulating factor antibodyCSF 2 antibodyCSF Alpha antibodyCSF antibodyCSF2 antibodyCSF2_HUMAN antibodyEosinophil Colony Stimulating Factor antibodyGM-CSF antibodyGMCSF antibodyGranulocyte Macrophage Colony Stimulating Factor antibodyGranulocyte-macrophage colony-stimulating factor antibodyMGC131935 antibodyMGC138897 antibodyMolgramostin antibodyPluripoietin Alpha antibodySargramostim antibody
      see all

    References for Anti-GM-CSF antibody [MP1-31G6] (ab13789)

    This product has been referenced in:
    • Hasegawa-Ishii S  et al. Defects in cytokine-mediated neuroprotective glial responses to excitotoxic hippocampal injury in senescence-accelerated mouse. Brain Behav Immun : (2010). IHC-FrFl ; Mouse . Read more (PubMed: 20804842) »

    See 1 Publication for this product

    Publishing research using ab13789 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab13789 Submit a Question for ab13789

    Displaying 1 - 5 of 5 results for Abreviews and Q&A

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    Customer kindly contacted us regarding the availability of an Igg1 isotype control for ab13789


    Our Rat IgG1, kappa monoclonal Isotype control is a suitable isotype control for ab13789 and has been tested and guaranteed to work in IHC-frozen. A link for this isotype control is given below:
    ab18412 (http://www.abcam.com/Rat-IgG1-kappa-monocl...

    Read More

    What is the recommended positive control tissue? Can I receive this antibody free of charge since ab7368 was not validated for frozen sections as previously indicated on the datasheet?

    Thank you very much for your call this afternoon.

    I'm currently looking through some publications and I'll get back to you shortly. Please let me know if you need anything else in the meantime.

    What is the recommended positive control tissue?

    Thank you for your phone call.


    The positive control information (lung) I gave you was for WB, not IHC. I am currently in contact with the lab to get some additional information on IHC images.

    I will forward this to you as soon as p...

    Read More

    Hello, I have been using the rat-anti-mouse-GM-CSF Ab for Western Blot with an anti-rat-HRP secondary Ab. Unfortunately, I was not able to even detect recombinant GM-CSF I was loading as a control. Looking at the datasheet of MP1-31G and the exemplar...

    Read More

    Thanks for you inquiry. I talked with the lab and they confirmed that ab13789 was raised in rat. Therefore, you should use an anti-rat secondary. It's possible that the WB image on the datasheet is in error. I contacted our datasheet people about t...

    Read More

    Western blot abreview for Anti-GM-CSF antibody [MP1-31G6]

     Good
    Abreviews
    Application Western blot
    Sample Mouse Tissue lysate - other (Lungs)
    Loading amount 20 µg
    Specification Lungs
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Read More
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    Verified customer

    Submitted Dec 12 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"