Overview

  • Product nameAnti-GM-CSF antibody [MP1-31G6]See all GM-CSF primary antibodies ...
  • Description
    Rat monoclonal [MP1-31G6] to GM-CSF
  • Tested applicationsIP, ELISA, Measuring secreted proteins from cells, WB, IHC-P more details
  • Species reactivity
    Reacts with: Mouse

    Does not react with

    Rat
  • Immunogen

    Recombinant full length protein (Mouse) expressed in yeast.

  • General notesAb13789 (for capturing) can be used for Sandwich ELISA with clone MP1-31G6 ab13788, ab45453, ab45470 (for detection).

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is affinity purified.
  • Clonality Monoclonal
  • Clone numberMP1-31G6
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab13789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP
ELISA
ELISPOT
WB
IHC-P
  • Application notesELISA: Use at an assay dependent dilution.
    ELISPOT: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml, this concentration of antibody can detect less than 100 ng of recombinant mouse GM-CSF under reducing conditions. Predicted molecular weight: 17.2 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
    • Sequence similaritiesBelongs to the GM-CSF family.
    • Cellular localizationSecreted.
    • Target information above from: UniProt accession P04141 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • Burst Promoting Activity antibody
      • CMCSF antibody
      • Colony stimulating factor 2 (granulocyte-macrophage) antibody
      • Colony Stimulating Factor 2 antibody
      • Colony stimulating factor antibody
      • Colony-stimulating factor antibody
      • CSF 2 antibody
      • CSF Alpha antibody
      • CSF antibody
      • CSF2 antibody
      • CSF2_HUMAN antibody
      • Eosinophil Colony Stimulating Factor antibody
      • GM-CSF antibody
      • GMCSF antibody
      • Granulocyte Macrophage Colony Stimulating Factor antibody
      • Granulocyte-macrophage colony-stimulating factor antibody
      • MGC131935 antibody
      • MGC138897 antibody
      • Molgramostin antibody
      • Pluripoietin Alpha antibody
      • Sargramostim antibody
      see all

    Anti-GM-CSF antibody [MP1-31G6] images

    • ab13789 staining GM-CSF in Mouse brain tissue sections by Immunohistochemistry - free floating. Antigen retrieval was by heat mediation in a citrate buffer pH 6. Samples were incubated with primary antibody (1/50) for 3 days. An Alexa Fluor 488-conjugated anti-rat IgG was used as the secondary antibody.

    References for Anti-GM-CSF antibody [MP1-31G6] (ab13789)

    This product has been referenced in:
    • Hasegawa-Ishii S  et al. Defects in cytokine-mediated neuroprotective glial responses to excitotoxic hippocampal injury in senescence-accelerated mouse. Brain Behav Immun : (2010). IHC-FrFl ; Mouse . Read more (PubMed: 20804842) »

    See 1 Publication for this product

    Product Wall


    Our Rat IgG1, kappa monoclonal Isotype control is a suitable isotype control for ab13789 and has been tested and guaranteed to work in IHC-frozen. A link for this isotype control is given below:
    ab18412 (http://www.abcam.com/Rat-IgG1-kappa-mon...

    Read More

    Thank you very much for your call this afternoon.

    I'm currently looking through some publications and I'll get back to you shortly. Please let me know if you need anything else in the meantime.

    Thank you for your phone call.


    The positive control information (lung) I gave you was for WB, not IHC. I am currently in contact with the lab to get some additional information on IHC images.

    I will forward this to you as soon a...

    Read More

    Thanks for you inquiry. I talked with the lab and they confirmed that ab13789 was raised in rat. Therefore, you should use an anti-rat secondary. It's possible that the WB image on the datasheet is in error. I contacted our datasheet people abou...

    Read More
    Application Western blot
    Sample Mouse Tissue lysate - other (Lungs)
    Loading amount 20 µg
    Specification Lungs
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 12 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"