Overview
- Product nameAnti-GM-CSF antibodySee all GM-CSF primary antibodies ...
- DescriptionRabbit polyclonal to GM-CSF
- Tested applicationsWB more details
- Species reactivityReacts with: Human
Predicted to work with: Rat, Rhesus monkey - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human GM-CSF.
(Peptide available as ab88237.)
- Positive controlThis antibody gave a positive signal in Human thymus tissue lysate as well as the following whole cell lysates: Jurkat; THP1; U937; HL60; HeLa.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab77768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 16 kDa).Can be blocked with GM-CSF peptide (ab88237). |
Target
- FunctionCytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
- Sequence similaritiesBelongs to the GM-CSF family.
- Cellular localizationSecreted.
-
Database links
- Entrez Gene: 1437 Human
- Entrez Gene: 116630 Rat
- Omim: 138960 Human
- SwissProt: P04141 Human
- SwissProt: P48750 Rat
- Unigene: 1349 Human
- Unigene: 44285 Rat
Target information above from: UniProt accession
P04141
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- Burst Promoting Activity antibodyCMCSF antibodyColony stimulating factor 2 (granulocyte-macrophage) antibody
- Colony Stimulating Factor 2 antibodyColony stimulating factor antibodyColony-stimulating factor antibodyCSF 2 antibodyCSF Alpha antibodyCSF antibodyCSF2 antibodyCSF2_HUMAN antibodyEosinophil Colony Stimulating Factor antibodyGM-CSF antibodyGMCSF antibodyGranulocyte Macrophage Colony Stimulating Factor antibodyGranulocyte-macrophage colony-stimulating factor antibodyMGC131935 antibodyMGC138897 antibodyMolgramostin antibodyPluripoietin Alpha antibodySargramostim antibody
see all
Anti-GM-CSF antibody images
-
Western blot - GM-CSF antibody (ab77768)All lanes : Anti-GM-CSF antibody (ab77768) at 1 µg/ml
Lane 1 :Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 4 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
Lane 5 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 16 kDa
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
Additional bands at : 77 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 12 minutes
References for Anti-GM-CSF antibody (ab77768)
ab77768 has not yet been referenced specifically in any publications.
