Overview

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab77768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 16 kDa).Can be blocked with Human GM-CSF peptide (ab88237).

Target

  • FunctionCytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • Sequence similaritiesBelongs to the GM-CSF family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Burst Promoting Activity antibody
    • CMCSF antibody
    • Colony stimulating factor 2 (granulocyte-macrophage) antibody
    • Colony Stimulating Factor 2 antibody
    • Colony stimulating factor antibody
    • Colony-stimulating factor antibody
    • CSF 2 antibody
    • CSF Alpha antibody
    • CSF antibody
    • CSF2 antibody
    • CSF2_HUMAN antibody
    • Eosinophil Colony Stimulating Factor antibody
    • GM-CSF antibody
    • GMCSF antibody
    • Granulocyte Macrophage Colony Stimulating Factor antibody
    • Granulocyte-macrophage colony-stimulating factor antibody
    • MGC131935 antibody
    • MGC138897 antibody
    • Molgramostin antibody
    • Pluripoietin Alpha antibody
    • Sargramostim antibody
    see all

Anti-GM-CSF antibody images

  • All lanes : Anti-GM-CSF antibody (ab77768) at 1 µg/ml

    Lane 1 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 4 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
    Lane 5 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
    Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 16 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 77 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References for Anti-GM-CSF antibody (ab77768)

ab77768 has not yet been referenced specifically in any publications.

Product Wall

Merci de nous avoir contactés.

Je suis ravi que l'anti-GM-CSF ab77768 fonctionne comme attendu.

Je conseillerais pour le contrôle isotypele polyclonal de lapin ab27478, http://www.abcam.com/ab27478.

Il est int...

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According to our records, ab54429, ab25123, ab1420 and ab8098 was proving difficult to use and we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting more details in order to help us be...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"