Directly conjugated antibodies for immunofluorescence

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Overview

  • Product nameAnti-GM-CSF antibody
    See all GM-CSF primary antibodies
  • Description
    Rabbit polyclonal to GM-CSF
  • Tested applicationsWB, ELISA, Neutralising, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Highly pure (>98%) recombinant mGM-CSF (mouse Granulocyte Macrophage Colony Stimulating Factor)

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.

Properties

  • FormLyophilised:Reconstitute with 200µl of sterile water.
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • Isotypeunknown
  • Light chain typeunknown
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab9741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect mGM-CSF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mGM-CSF.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of mGM-CSF (0.6 ng/ml), a concentration of 0.012 - 0.02 µg/ml of this antibody is required.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionCytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • Sequence similaritiesBelongs to the GM-CSF family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • Burst Promoting Activity antibody
      • CMCSF antibody
      • Colony stimulating factor 2 (granulocyte-macrophage) antibody
      • Colony Stimulating Factor 2 antibody
      • Colony stimulating factor antibody
      • Colony-stimulating factor antibody
      • CSF 2 antibody
      • CSF Alpha antibody
      • CSF antibody
      • CSF2 antibody
      • CSF2_HUMAN antibody
      • Eosinophil Colony Stimulating Factor antibody
      • GM-CSF antibody
      • GMCSF antibody
      • Granulocyte Macrophage Colony Stimulating Factor antibody
      • Granulocyte-macrophage colony-stimulating factor antibody
      • MGC131935 antibody
      • MGC138897 antibody
      • MGI1GM antibody
      • Molgramostin antibody
      • Pluripoietin Alpha antibody
      • Pluripoietin-a antibody
      • Sargramostim antibody
      see all

    Anti-GM-CSF antibody images

    • Western blot - Anti-GM-CSF antibody (ab9741)
      Western blot - Anti-GM-CSF antibody (ab9741)


      Performed under reducing conditions.

      Observed band size : 17 kDa (why is the actual band size different from the predicted?)

      Western blot analysis of recombinant GM-CSF using ab9741 at 0.1μg/ml. Bottom band - dye front.

    • Western blot - Anti-GM-CSF antibody (ab9741)
      Western blot - Anti-GM-CSF antibody (ab9741)


      Performed under non-reducing conditions.

      Observed band size : 17 kDa (why is the actual band size different from the predicted?)

      Western blot analysis of recombinant GM-CSF using ab9741 at 0.1μg/ml. Bottom band - dye front.

    • ELISA - Anti-GM-CSF antibody (ab9741)
      ELISA - Anti-GM-CSF antibody (ab9741)
      Less than 0.10 ug/ml ab9741 will neutralize 50% of the bioactivity of 1 ng/ml m-GM-CSF.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM-CSF antibody (ab9741)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM-CSF antibody (ab9741)

      IHC image of GM-CSF staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9741, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References for Anti-GM-CSF antibody (ab9741)

    ab9741 has not yet been referenced specifically in any publications.

    Publishing research using ab9741 ? Please let us know so that we can cite the reference in this datasheet

    Related Products

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    Submit an abreview for ab9741 Submit a question for ab9741

    5 Abreviews or Q&A

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    Votes: Highest First

    Inquiry: Dear Sir/ Madam, Sorry to disturb you. Our customer raised an enquiry about ab9741. She already used ab9741 to conduct the ICC/IF assay with primary culture cell--Rat Cerebral Cortex tissue. She thinks the expression of GM-CSF protein should i...

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    Thank you for contacting us.

    ab9741 has not been tested in ICC/IF so we do not have any useful information to provide the customer.

    Please do not hesitate to contact us if you need any more advice or information.

    Use our ...

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    Dear Tech support

    One of our customers is interested in inhibiting cytokine mediated immune responses in Human and Mouse cell lines.
    The customer has asked the following questions regarding products ab9741, ab64715:

    What is t...

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    Thank you for contacting us and sorry for the delay in getting back to you.

    The neutralizing and inhibition functions stated on the datasheet of the antibodies ab9741 andab64715 relate to the assays which have been performed to validate their...

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    Inquiry: Dear Sir/ Madam, Our customer raise an inquiry about Anti-GM-CSF antibody (ab9741). She would like to test in Rat/ WB. Besides, she would like to know the percentage of sequence homology between rat sequence and mouse immunogen.
    Woul...

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    Thank you very much for your inquiry.

    The immunogen of ab9741 was the whole GM-CSFprotein of mouse. Mouse GM-CSF only shows 71% homology with rat as a whole protein and therefore I cannot recommend to use this antibody for rat samples.
    <...

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    Dear Sir/Madam, I want to know antibody(ab9741) in WB experiments should be location at which what molecular weight?

    Thank you for contacting us with your question.
    The GM-CSF protein is expected to run around 16 kDa, however there are 4 known glycosylation sites, each adding around 2 kDa to the protein, so the protein generally runs around 24 kDa on a Western b...

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    ANTIBODY CODE ab9741 BATCH NUMBER 19349 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Multiple bands, we are finding bands at just above 20, and two bands between 37-50 kD. these higher molecular weight bands a...

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    Unfortunately, we only test against the recombinant murine GM-CSF and do not test tissue samples. In the naturally occurring GM-CSF there are potential glycolisation sites which could cause the protein to migrate higher on a WB. Sorry we coul...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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