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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
Overview
Product name
Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker
See all GM130 products (7) ...
Description
Rabbit polyclonal to GM130 - Drosophila Golgi/Cis-Golgi Marker
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Fruit fly (Drosophila melanogaster)
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Fruit fly (Drosophila melanogaster) GM130.
(Peptide available as ab32335.)
Positive control
Drosophila S2 Cells
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Golgi Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: Use a concentration of 0.1 µg/mlDetects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
Target
Function
Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.
Sequence similarities
Belongs to the GOLGA2 family.
Domain
Extended rod-like protein with coiled-coil domains.
Cellular localization
Golgi apparatus > Golgi stack membrane.
Target information above from: UniProt accessionQ08379
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 130 kDa cis Golgi matrix protein antibody
- 130 kDa cis-Golgi matrix protein antibody
- Cis golgi matrix protein GM130 antibody
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Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker images:
Immunocytochemistry - GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637)
The image shows staining of Drosophila S2 cells using ab30637 to cis-Golgi/Golgi marker, GM130. For comparison, S2 cells were stained with an antibody to the trans-Golgi marker, GCC88. Gm130 stained the cis-Golgi region and appears to be a superior Golgi marker to GCC88.
Sean Munro, MRC Laboratory of Molecular Biology
Western blot - GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637)
All lanes : Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 0.1 µg/ml
Lane 1 : Drosophila melanogaster S2 cell extract
Lane 2 : Drosophila melanogaster S2 cell extract + specific siRNA
Predicted band size : 95 kDa
Observed band size : 95 kDa
Sean Munro, MRC Laboratory of Molecular Biology
References for Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637)
This product has been referenced in:
- Zhou Xet al. Auxilin is required for formation of Golgi-derived clathrin-coated vesicles during Drosophila spermatogenesis. Development 138:1111-20 (2011).Read more (PubMed: 21343365) »
- Zhang Let al. An O-glycosyltransferase promotes cell adhesion during development by influencing secretion of an extracellular matrix integrin ligand. J Biol Chem 285:19491-501 (2010). ICC/IF; Fruit fly (Drosophila melanogaster).Read more (PubMed: 20371600) »
See all 8 publications for this product
Publishing research using ab30637? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"