Western blot - GM130 antibody [EP892Y] (ab52649)

Anti-GM130 antibody [EP892Y] (ab52649) at 1/200000 dilution + 10 µg of HeLa cell lysate

Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size : 112 kDa

Immunohistochemistry (Paraffin-embedded sections) - GM130 antibody [EP892Y] (ab52649)

ab52649 (1/500) staining GM130 in paraffin-embedded Human liver tissue sections.

Immunocytochemistry/ Immunofluorescence - GM130 antibody [EP892Y] (ab52649)

ICC/IF image of ab52649 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52649, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunocytochemistry/ Immunofluorescence - GM130 antibody [EP892Y] (ab52649)

ab52649 staining GM130 in human ARPE-19 cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were formaldehyde fixed, permeabilized by 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25ºC. The sample was incubated with the primary antibody (1/500 in 1% goat serum, 0.1%TX100, 1 x PBS) for 16 hours at 4ºC. An Alexa Fluor^®488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary.

This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic

Immunocytochemistry/ Immunofluorescence - GM130 antibody [EP892Y] (ab52649)

ab52649 staining GM130 in Bovine brain microvascular endothelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 5% BSA for 90 minutes at 37ºC. Samples were incubated with primary antibody (1/100 in 0.1% saponin + 1% BSA ) for 18 hours at 4ºC. An undiluted Alexa Fluor^®568-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

This image is courtesy of an Abreview submitted by JL Balligand

Immunocytochemistry/ Immunofluorescence - GM130 antibody [EP892Y] (ab52649)

ICC/IF image of ab52946 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52946, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.