You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299)

CodeSizePriceAbpointsAvailability
    
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-GM130 antibody [NN 2C10/1] - Golgi Marker
See all GM130 products (7) ...

Description

Mouse monoclonal [NN 2C10/1] to GM130 - Golgi Marker

Tested applications

ICC/IF, IHC-P, WB, IHC-FoFr, Electron Microscopymore details

Cross reactivity

Reacts with

Mouse, Rat, Chinese Hamster

Does not react with

Human

Immunogen

Recombinant full length protein (Rat).

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Phosphate buffered saline

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

NN 2C10/1

Myeloma

Sp2

Isotype

IgG2a

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299)Immunohistochemistry (PFA perfusion fixed frozen sections) - GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299)Immunocytochemistry/ Immunofluorescence - GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab1299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.

Sequence similarities

Belongs to the GOLGA2 family.

Domain

Extended rod-like protein with coiled-coil domains.

Cellular localization

Golgi apparatus > Golgi stack membrane.

Target information above from: UniProt accessionQ08379 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 130 kDa cis Golgi matrix protein antibody
  • 130 kDa cis-Golgi matrix protein antibody
  • Cis golgi matrix protein GM130 antibody
  • GM130 antibody
  • GM130 autoantigen antibody
  • GOGA2_HUMAN antibody
  • GOLGA 2 antibody
  • GOLGA2 antibody
  • Golgi autoantigen antibody
  • Golgi autoantigen golgin subfamily a 2 antibody
  • Golgi matrix protein GM130 antibody
  • Golgin 95 antibody
  • golgin A2 antibody
  • Golgin subfamily a 2 antibody
  • Golgin subfamily A member 2 antibody
  • Golgin-95 antibody
  • MGC20672 antibody
  • SY11 protein antibody
see all

Anti-GM130 antibody [NN 2C10/1] - Golgi Marker images:

  Immunohistochemistry (PFA perfusion fixed frozen sections) - GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299)

Immunohistochemistry (PFA perfusion fixed frozen sections) - GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299)

ab1299 staining GM130 in Fruitfly epidermal cell tissue by Immunohistochemistry (PFA perfusion fixed frozen sections).

Image from Norum M et al, PLoS One. 2010 May 24;5(5):e10802, Fig 8.

  Immunocytochemistry/ Immunofluorescence - GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299)

Immunocytochemistry/ Immunofluorescence - GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299)

ab1299 staining GM130 in Chinese hamster ovary cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X and then blocked using 10% FCS for 30 minutes at room temperature. Samples were then incubated with primary antibody at 1/100 for 2 hours. The secondary antibody used was a goat IgG conjugated to Alexa Fluor® 488 (green) used at a 1/1000 dilution.

Image courtesy of an anonymous Abreview.

See Abreview

References for Anti-GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299)

This product has been referenced in:

  • Yang Met al. Precursor of Brain-derived Neurotrophic Factor (proBDNF) Forms a Complex with Huntingtin-associated Protein-1 (HAP1) and Sortilin That Modulates proBDNF Trafficking, Degradation, and Processing. J Biol Chem 286:16272-84 (2011). ICC/IF; Mouse.Read more (PubMed: 21357693) »
  • Norum Met al. Trafficking through COPII stabilises cell polarity and drives secretion during Drosophila epidermal differentiation. PLoS One 5:e10802 (2010). IHC-FoFr; Fruit fly (Drosophila melanogaster).Read more (PubMed: 20520821) »

See all 12 publications for this product

Publishing research using ab1299? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"