GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299)

Product Name 

GM130 antibody [NN 2C10/1] - Golgi Marker

Product type 

Primary antibodies

Description 

Mouse monoclonal [NN 2C10/1] to GM130 - Golgi Marker

Immunogen 

Recombinant full length protein (Rat).

Reacts with 

Rat

Does not react with 
(species key)

Hu

Tested applications 
(see key)

EM, ICC/IF, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
EM: Use at an assay dependent dilution (PMID 19015319).
ICC/IF: 1/200 (PMID 19022387).
IHC-P: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Peripheral membrane protein associated with Golgi stacks

Research areas 

Signal Transduction >> Protein Trafficking >> Golgi Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi

Relevance 

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. GM130 encodes a member of the Golgin protein family and is localised to the Golgi. The protein encoded by GM130 has been postulated to play roles in the stacking of Golgi cisternae, maintaining cis-Golgi structure and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. GM130 is part of a larger oligomeric complex which interacts with p115, GORASP1/GRASP65 and RAB1B that has been activated by GTP-binding. GM130 is useful as a cis-Golgi marker in immunohistochemistry.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

NN 2C10/1

Myeloma 

Sp2

Isotype 

IgG2a

Purity 

Protein A purified

Storage buffer 

Phosphate buffered saline

Form 

Liquid

Concentration 

0.750 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299)

  This product has been used in: (two most recent references)

Merianda TT et al. A functional equivalent of endoplasmic reticulum and Golgi in axons for secretion of locally synthesized proteins. Mol Cell Neurosci 40:128-42 (2009). ICC/IF; Rat. PubMed: 19022387

Li Y et al. HUMMR, a hypoxia- and HIF-1alpha-inducible protein, alters mitochondrial distribution and transport. J Cell Biol 185:1065-81 (2009). ICC/IF; Mouse. PubMed: 19528298

See all 10 publication references for this product.


If you publish research using ab1299 please let us know so that we can cite the reference on this datasheet.

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