Feedback Form

Product Name 

GM130 antibody [NN 2C10/1] - Golgi Marker

 

See all GM130 - Golgi Marker antibodies (6)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [NN 2C10/1] to GM130 - Golgi Marker

Immunogen 

Recombinant full length protein (Rat).

Reacts with 

Rat

Does not react with 
(species key)

Hu

Tested applications 
(see key)

EM, ICC/IF, IHC-P, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews

Submit an Abreview

Be the first to review this antibody and earn 220 Abpoints.


Application notes 
(see key)

Recommended dilutions
EM: Use at an assay dependent dilution (PMID 19015319).
ICC/IF: 1/200 (PMID 19022387).
IHC-P: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Peripheral membrane protein associated with Golgi stacks

Research areas 

Signal Transduction >> Protein Trafficking >> Golgi Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi

Relevance 

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. GM130 encodes a member of the Golgin protein family and is localised to the Golgi. The protein encoded by GM130 has been postulated to play roles in the stacking of Golgi cisternae, maintaining cis-Golgi structure and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. GM130 is part of a larger oligomeric complex which interacts with p115, GORASP1/GRASP65 and RAB1B that has been activated by GTP-binding. GM130 is useful as a cis-Golgi marker in immunohistochemistry.

 

Alternative names GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

64528    (Rat)

SwissProt

    

Q62839    (Rat)

Unigene

    

6165    (Rat)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

NN 2C10/1

Myeloma 

Sp2

Isotype 

IgG2a

Purity 

Protein A purified

Storage buffer 

Phosphate buffered saline

Form 

Liquid

Concentration 

0.750 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GM130 antibody [NN 2C10/1] - Golgi Marker is on this datasheet. But please do contact us if you would like any reassurance!



References for GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299)

Specific publication references   This product has been used in: (two most recent references)

Merianda TT et al. A functional equivalent of endoplasmic reticulum and Golgi in axons for secretion of locally synthesized proteins. Mol Cell Neurosci 40:128-42 (2009). ICC/IF; Rat. PubMed: 19022387

Li Y et al. HUMMR, a hypoxia- and HIF-1alpha-inducible protein, alters mitochondrial distribution and transport. J Cell Biol 185:1065-81 (2009). ICC/IF; Mouse. PubMed: 19528298


See all 10 publication references for this product.


If you publish research using ab1299 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to GM130

 

GM130 antibody [NN 2C10/1] - Golgi Marker - more information

Customer reviews (feedback) regarding GM130 antibody [NN 2C10/1] - Golgi Marker

Customer FAQs regarding GM130 antibody [NN 2C10/1] - Golgi Marker

Protocols for GM130 antibody [NN 2C10/1] - Golgi Marker

Price and availability of products related to GM130 antibody [NN 2C10/1] - Golgi Marker

 

GM130 antibody [NN 2C10/1] - Golgi Marker - related products:

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

Products for Western blot

Loading controls

Lysates

 

Compatible Secondaries for GM130 antibody [NN 2C10/1] - Golgi Marker

ab2891

  

HRP polymer antibody

ab6563

  

Cy5 ® antibody

ab6726

  

Texas Red ® antibody

ab6728

  

HRP antibody

ab6729

  

Alkaline Phosphatase antibody

ab6785

  

FITC antibody

ab6788

  

Biotin antibody

ab7002

  

Phycoerythrin antibody

ab7018

  

Agarose antibody

ab27229

  

15nm Gold antibody

ab27230

  

10nm Gold antibody

ab27231

  

15nm Gold antibody

ab27233

  

5nm Gold antibody

ab27241

  

10nm Gold antibody

ab27242

  

20nm Gold antibody

ab27243

  

40nm Gold antibody

ab27244

  

5nm Gold antibody

ab60313

  

Chromeo™ 488 antibody

ab60316

  

Chromeo™ 546 antibody

ab60318

  

Chromeo™ 642 antibody

 

Other GM130 Antibodies

See all GM130 - Golgi Marker antibodies (6)...

 

GM130 antibody [NN 2C10/1] - Golgi Marker - other tools:

Contact Abcam with a Technical Enquiry about GM130 antibody [NN 2C10/1] - Golgi Marker

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.