![Immunohistochemistry (PFA perfusion fixed frozen sections) - GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299) image](#GM130-Primary-antibodies-ab1299-1.jpg)
![Immunocytochemistry/ Immunofluorescence - GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299) image](#GM130-Primary-antibodies-ab1299-4.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
Overview
Product name
Anti-GM130 antibody [NN 2C10/1] - Golgi Marker
See all GM130 products (7) ...
Description
Mouse monoclonal [NN 2C10/1] to GM130 - Golgi Marker
Tested applications
ICC/IF, IHC-P, WB, IHC-FoFr, Electron Microscopymore details
Cross reactivity
Reacts with
Mouse, Rat, Chinese Hamster
Does not react with
Human
Immunogen
Recombinant full length protein (Rat).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Phosphate buffered saline
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
NN 2C10/1
Myeloma
Sp2
Isotype
IgG2a
Research areas
Signal Transduction >> Protein Trafficking >> Golgi Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/200(PubMed: 19022387)
IHC-P: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 20520821)
EM: Use at an assay dependent dilution. (PubMed: 19015319)
Target
Function
Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.
Sequence similarities
Belongs to the GOLGA2 family.
Domain
Extended rod-like protein with coiled-coil domains.
Cellular localization
Golgi apparatus > Golgi stack membrane.
Target information above from: UniProt accessionQ08379
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 130 kDa cis Golgi matrix protein antibody
- 130 kDa cis-Golgi matrix protein antibody
- Cis golgi matrix protein GM130 antibody
see all
Database links
- Entrez Gene: 99412 Mouse
- Entrez Gene: 64528 Rat
- SwissProt: Q921M4 Mouse
- SwissProt: Q62839 Rat
- Unigene: 106376 Mouse
- Unigene: 6165 Rat
Anti-GM130 antibody [NN 2C10/1] - Golgi Marker images:
Immunohistochemistry (PFA perfusion fixed frozen sections) - GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299)
![Immunohistochemistry (PFA perfusion fixed frozen sections) - GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299)](/ps/datasheet/images/1/ab1299/GM130-Primary-antibodies-ab1299-1.jpg)
ab1299 staining GM130 in Fruitfly epidermal cell tissue by Immunohistochemistry (PFA perfusion fixed frozen sections).
Image from Norum M et al, PLoS One. 2010 May 24;5(5):e10802, Fig 8.
Immunocytochemistry/ Immunofluorescence - GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299)
![Immunocytochemistry/ Immunofluorescence - GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299)](/ps/datasheet/images/1/ab1299/GM130-Primary-antibodies-ab1299-4.jpg)
ab1299 staining GM130 in Chinese hamster ovary cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X and then blocked using 10% FCS for 30 minutes at room temperature. Samples were then incubated with primary antibody at 1/100 for 2 hours. The secondary antibody used was a goat IgG conjugated to Alexa Fluor® 488 (green) used at a 1/1000 dilution.
Image courtesy of an anonymous Abreview.
References for Anti-GM130 antibody [NN 2C10/1] - Golgi Marker (ab1299)
This product has been referenced in:
- Yang Met al. Precursor of Brain-derived Neurotrophic Factor (proBDNF) Forms a Complex with Huntingtin-associated Protein-1 (HAP1) and Sortilin That Modulates proBDNF Trafficking, Degradation, and Processing. J Biol Chem 286:16272-84 (2011). ICC/IF; Mouse.Read more (PubMed: 21357693) »
- Norum Met al. Trafficking through COPII stabilises cell polarity and drives secretion during Drosophila epidermal differentiation. PLoS One 5:e10802 (2010). IHC-FoFr; Fruit fly (Drosophila melanogaster).Read more (PubMed: 20520821) »
See all 12 publications for this product
Publishing research using ab1299? Please let us know so that we can cite the reference in this datasheet
![GM130 antibody [NN 2C10/1] - Golgi Marker for Immunocytochemistry/ Immunofluorescence in Chinese Hamster (1299)](/ps/datasheet/images/1/ab1299/GM130-Primary-antibodies-ab1299-3.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"