Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
Overview
Product name
Anti-GM130 antibody
See all GM130 products (7) ...
Description
Rabbit polyclonal to GM130
Tested applications
Cross reactivity
Predicted to work with
Mouse, Rat, Human
Immunogen
Synthetic peptide derived from within residues 950 to the C-terminus of Rat GM130.
Positive control
This antibody gave a positive signal in the following Human whole cell lysates: HeLa; MCF7.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Golgi Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 130 kDa (predicted molecular weight: 111 kDa).
Target
Function
Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.
Sequence similarities
Belongs to the GOLGA2 family.
Domain
Extended rod-like protein with coiled-coil domains.
Cellular localization
Golgi apparatus > Golgi stack membrane.
Target information above from: UniProt accessionQ08379
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 130 kDa cis Golgi matrix protein antibody
- 130 kDa cis-Golgi matrix protein antibody
- Cis golgi matrix protein GM130 antibody
see all
Database links
- Entrez Gene: 2801 Human
- Entrez Gene: 99412 Mouse
- Entrez Gene: 64528 Rat
- Omim: 602580 Human
- SwissProt: Q08379 Human
- SwissProt: Q6GRM9 Human
- SwissProt: Q921M4 Mouse
- SwissProt: Q62839 Rat
see all
Anti-GM130 antibody images:
Immunocytochemistry/ Immunofluorescence - GM130 antibody (ab31561)
ICC/IF image of ab31561 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab31561, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Western blot - GM130 antibody (ab31561)
All lanes : Anti-GM130 antibody (ab31561) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 111 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Exposure time : 4 minutes
References for Anti-GM130 antibody (ab31561)
ab31561 has not yet been referenced specifically in any publications.
Publishing research using ab31561? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"