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Western blot - GM130 antibody (ab32337)
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Product Name
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GM130 antibody
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See all GM130 - Golgi Marker antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to GM130
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Fruit fly (Drosophila melanogaster) GM130. (Peptide available as ab32336.)
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Reacts with
(species key)
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Dm
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Drosophila S2 Lysate.
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Cellular localization
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Peripheral membrane protein associated with Golgi stacks
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Research areas
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Signal Transduction >> Protein Trafficking >> Golgi Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
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Relevance
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The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. GM130 encodes a member of the Golgin protein family and is localised to the Golgi. The protein encoded by GM130 has been postulated to play roles in the stacking of Golgi cisternae, maintaining cis-Golgi structure and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. GM130 is part of a larger oligomeric complex which interacts with p115, GORASP1/GRASP65 and RAB1B that has been activated by GTP-binding. GM130 is useful as a cis-Golgi marker in immunohistochemistry.
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Database links
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The links below go to external sites and will open in a new browser window NCBI | | CAB77666 (Fruit fly (Drosophila melanogaster))
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.16 - 0.50 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GM130 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GM130 antibody images, references, products related to ab32337 and other tools.
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GM130 antibody images:
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 Western blot - GM130 antibody (ab32337)
GM130 antibody (ab32337) at 1 µg/ml + Drosophila S2 Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 95 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to GM130
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GM130 antibody - more information
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Customer reviews (feedback) regarding GM130 antibody |
Customer FAQs regarding GM130 antibody |
Protocols for GM130 antibody |
Price and availability of products related to GM130 antibody |
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GM130 antibody - related products:
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GM130 antibody - other tools:
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Contact Abcam with a Technical Enquiry about GM130 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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