Anti-GM130 antibody (ab32337)
Overview
- Product nameAnti-GM130 antibodySee all GM130 primary antibodies ...
- DescriptionRabbit polyclonal to GM130
- Tested applicationsICC/IF, WB more details
- Species reactivityReacts with: Fruit fly (Drosophila melanogaster)
- Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Fruit fly (Drosophila melanogaster) GM130.
(Peptide available as ab32336.)
- Positive controlDrosophila S2 Lysate.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab32337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use at an assay dependent concentration. PubMed: 22543973 |
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).Can be blocked with GM130 peptide (ab32336). |
Target
- FunctionGolgi auto-antigen; probably involved in maintaining cis-Golgi structure.
- Sequence similaritiesBelongs to the GOLGA2 family.
- DomainExtended rod-like protein with coiled-coil domains.
- Cellular localizationGolgi apparatus > Golgi stack membrane.
Target information above from: UniProt accession
Q08379
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 130 kDa cis Golgi matrix protein antibody130 kDa cis-Golgi matrix protein antibodyCis golgi matrix protein GM130 antibody
- GM130 antibodyGM130 autoantigen antibodyGOGA2_HUMAN antibodyGOLGA 2 antibodyGOLGA2 antibodyGolgi autoantigen antibodyGolgi autoantigen golgin subfamily a 2 antibodyGolgi matrix protein GM130 antibodyGolgin 95 antibodygolgin A2 antibodyGolgin subfamily a 2 antibodyGolgin subfamily A member 2 antibodyGolgin-95 antibodyMGC20672 antibodySY11 protein antibody
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Anti-GM130 antibody images
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Western blot - GM130 antibody (ab32337)Anti-GM130 antibody (ab32337) at 1 µg/ml + Drosophila S2 Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 95 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
References for Anti-GM130 antibody (ab32337)
This product has been referenced in:
- O'Sullivan NC et al. Reticulon-like-1, the Drosophila orthologue of the Hereditary Spastic Paraplegia gene reticulon 2, is required for organization of endoplasmic reticulum and of distal motor axons. Hum Mol Genet 21:3356-65 (2012). ICC/IF ; Fruit fly (Drosophila melanogaster) . Read more (PubMed: 22543973) »
