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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
Overview
Product name
Anti-GM130 antibody
See all GM130 products (7) ...
Description
Rabbit polyclonal to GM130
Tested applications
Cross reactivity
Reacts with
Fruit fly (Drosophila melanogaster)
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Fruit fly (Drosophila melanogaster) GM130.
(Peptide available as ab32336.)
Positive control
Drosophila S2 Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Golgi Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
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Western blot - GM130 antibody (ab32337)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
Target
Function
Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.
Sequence similarities
Belongs to the GOLGA2 family.
Domain
Extended rod-like protein with coiled-coil domains.
Cellular localization
Golgi apparatus > Golgi stack membrane.
Target information above from: UniProt accessionQ08379
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 130 kDa cis Golgi matrix protein antibody
- 130 kDa cis-Golgi matrix protein antibody
- Cis golgi matrix protein GM130 antibody
see all
Anti-GM130 antibody images:
Western blot - GM130 antibody (ab32337)
Anti-GM130 antibody (ab32337) at 1 µg/ml + Drosophila S2 Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 95 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
References for Anti-GM130 antibody (ab32337)
ab32337 has not yet been referenced specifically in any publications.
Publishing research using ab32337? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"