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Western blot - GM130 antibody (ab32337)
Western blot - GM130 antibody (ab32337)
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Product Name 

GM130 antibody

 

See all GM130 - Golgi Marker antibodies (6)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to GM130

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Fruit fly (Drosophila melanogaster) GM130.

(Peptide available as ab32336.)

Reacts with 
(species key)

Dm

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Drosophila S2 Lysate.

Cellular localization 

Peripheral membrane protein associated with Golgi stacks

Research areas 

Signal Transduction >> Protein Trafficking >> Golgi Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi

Relevance 

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. GM130 encodes a member of the Golgin protein family and is localised to the Golgi. The protein encoded by GM130 has been postulated to play roles in the stacking of Golgi cisternae, maintaining cis-Golgi structure and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. GM130 is part of a larger oligomeric complex which interacts with p115, GORASP1/GRASP65 and RAB1B that has been activated by GTP-binding. GM130 is useful as a cis-Golgi marker in immunohistochemistry.

 

Alternative names GM130 antibody (ab32337)

Database links 

The links below go to external sites and will open in a new browser window

NCBI

    

CAB77666    (Fruit fly (Drosophila melanogaster))

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 0.16 - 0.50 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GM130 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for GM130 antibody images, references, products related to ab32337 and other tools.

 

GM130 antibody images:

Product image  Western blot - GM130 antibody (ab32337)

Western blot - GM130 antibody (ab32337)

GM130 antibody (ab32337) at 1 µg/ml + Drosophila S2 Lysate at 10 µg

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 95 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

 
 

Search PubMed (MEDLINE) for references to GM130

 

GM130 antibody - more information

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Price and availability of products related to GM130 antibody

 

GM130 antibody - related products:

Products for Western blot

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Lysates

 

Immunizing Peptide for GM130 antibody

ab32336

  

GM130 peptide

 

Compatible Secondaries for GM130 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other GM130 Antibodies

See all GM130 - Golgi Marker antibodies (6)...

 

GM130 antibody - other tools:

Contact Abcam with a Technical Enquiry about GM130 antibody

 

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