Recombinant Anti-GNAI3 antibody [EPR11894] (ab173527)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11894] to GNAI3
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GNAI3 antibody [EPR11894]
See all GNAI3 primary antibodies -
Description
Rabbit monoclonal [EPR11894] to GNAI3 -
Host species
Rabbit -
Specificity
The peptide immunogen shares a 92.9% homology with GNAI1 and GNAI2, therefore we believe cross-reactivity is highly likely but this has not been experimentally assessed. -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Guinea pig, Cow, Xenopus laevis -
Immunogen
Synthetic peptide within Human GNAI3 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P08754 -
Positive control
- WB: Human fetal brain, fetal kidney, HepG2 and A431 lysates. Immunoprecipitation pellet from Human fetal brain lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11894 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab173527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa. |
IP
1/10 - 1/100. |
Target
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Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. -
Sequence similarities
Belongs to the G-alpha family. G(i/o/t/z) subfamily. -
Cellular localization
Cytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody. - Information by UniProt
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Database links
- Entrez Gene: 374097 Chicken
- Entrez Gene: 539521 Cow
- Entrez Gene: 2773 Human
- Entrez Gene: 14679 Mouse
- Entrez Gene: 25643 Rat
- Entrez Gene: 379236 Xenopus laevis
- Omim: 139370 Human
- SwissProt: P38403 Guinea pig
see all -
Alternative names
- 87U6 antibody
- FLJ26559 antibody
- G protein alpha inhibiting 3 antibody
see all
Images
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All lanes : Anti-GNAI3 antibody [EPR11894] (ab173527) at 1/1000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : Human fetal kidney lysates
Lane 3 : HepG2 lysates
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 41 kDa -
Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal brain lysate or (Lane 2) 1X PBS (negative control) using ab173527 at a 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab173527 has been referenced in 2 publications.
- Nusková H et al. Stearic acid blunts growth-factor signaling via oleoylation of GNAI proteins. Nat Commun 12:4590 (2021). PubMed: 34321466
- Freeman S et al. Proteostasis is essential during cochlear development for neuron survival and hair cell polarity. EMBO Rep 20:e47097 (2019). PubMed: 31321879