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Western blot - GNAQ+ GNA11 antibody (ab79337)
(enlarge)
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Western blot - GNAQ+ GNA11 antibody (ab79337)
(enlarge)
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Product Name
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GNAQ+ GNA11 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to GNAQ+ GNA11
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Immunogen
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Synthetic peptide: QLNLKEYNLV conjugated to KLH, corresponding to amino acids 350-359 of Human GNA11 or 344-353 of Human GNAQ and GNA11.
BLAST 'QLNLKEYNLV' with  or 
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Mamm
(Due to sequence homology)
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Specificity
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Specific for GNAQ and GNA11. It does not recognize other non-pertussis toxin-sensitive G proteins.
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at a concentration of 300 - 500 mg/ml.
WB: 1/500 - 1/2000. Predicted molecular weight: 42 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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NIH-3T3 cell membrane extract. Rat brain microsomal preparation.
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Cellular localization
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Cytoplasm. Plasma membrane.
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Research areas
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Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Heterotrimeric G Proteins >> G Proteins
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Relevance
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Guanine nucleotide-binding proteins are a family of heterotrimeric proteins that couple cell surface, 7-transmembrane domain receptors to intracellular signaling pathways. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit. GNAQ and GNA11 are alpha subunits of one of the heterotrimeric GTP-binding proteins and mediate stimulation of phospholipase C.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: PBS, pH 7.4
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GNAQ+ GNA11 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GNAQ+ GNA11 antibody images, references, products related to ab79337 and other tools.
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GNAQ+ GNA11 antibody images:
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 Western blot - GNAQ+ GNA11 antibody (ab79337)
GNAQ+ GNA11 antibody (ab79337) at 1/1000 dilution + NIH-3T3 cell membrane extract at 20 µg
Secondary
Goat anti-Rabbit HRP-conjugated IgG at 1/5000 dilution
Predicted band size : 42 kDa
Observed band size : 42 kDa
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Western blot - GNAQ+ GNA11 antibody (ab79337)
GNAQ+ GNA11 antibody (ab79337) at 1/2000 dilution + Rat brain microsomal preparation at 20 µg
Secondary
Goat anti-rabbit HRP-conjugated IgG at 1/5000 dilution
Predicted band size : 42 kDa
Observed band size : 42 kDa
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Search PubMed (MEDLINE) for references to GNAQ+ GNA11
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GNAQ+ GNA11 antibody - more information
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Customer reviews (feedback) regarding GNAQ+ GNA11 antibody |
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GNAQ+ GNA11 antibody - related products:
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GNAQ+ GNA11 antibody - other tools:
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Contact Abcam with a Technical Enquiry about GNAQ+ GNA11 antibody |
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