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Products:Cardiovascular >> Blood >> Coagulation >> Regulatory
Overview
Product name
Anti-GNAQ antibody
Description
Rabbit polyclonal to GNAQ
Specificity
The immunogen has 66% homology with GNA11
Tested applications
IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Dog, Pig, Xenopus laevis
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human GNAQ.
(Peptide available as ab86404.)
Positive control
This antibody gave a positive signal in the following lysates: HepG2 Whole Cell; Mouse Pancreas Tissue; Mouse Kidney Tissue; Rat Liver Tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Apoptosis
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Heterotrimeric G Proteins >> G Proteins
Signal Transduction >> Second Messenger >> Nucleotide Messenger >> GTP
Neuroscience >> Neurology process >> Neural Signal Transduction
Cardiovascular >> Blood >> Coagulation >> Regulatory
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
Tissue specificity
Predominantly expressed in ovary, prostate, testis and colon.
Sequence similarities
Belongs to the G-alpha family. G(q) subfamily.
Target information above from: UniProt accessionP50148
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- G ALPHA q antibody
- G protein alpha Q antibody
- GAQ antibody
see all
Database links
- Entrez Gene: 2776 Human
- Entrez Gene: 14682 Mouse
- Entrez Gene: 81666 Rat
- Entrez Gene: 394425 Xenopus laevis
- Entrez Gene: 399261 Xenopus laevis
- Omim: 600998 Human
- SwissProt: P50148 Human
- SwissProt: P21279 Mouse
see all
Anti-GNAQ antibody images:
Western blot - GNAQ antibody (ab75825)
All lanes : Anti-GNAQ antibody (ab75825) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Pancreas (Mouse) Tissue Lysate
Lane 3 : Kidney (Mouse) Tissue Lysate
Lane 4 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP)
developed using the ECL technique
Predicted band size : 42 kDa
Observed band size : 42 kDa
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - GNAQ antibody (ab75825)
ICC/IF image of ab75825 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75825, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GNAQ antibody (ab75825)
IHC image of G protein alpha q staining in human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75825, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
References for Anti-GNAQ antibody (ab75825)
This product has been referenced in:
- Mak Set al. Differential expression of genes in the calcium-signaling pathway underlies lesion development in the LDb mouse model of atherosclerosis. Atherosclerosis 213:40-51 (2010).Read more (PubMed: 20667539) »
See 1 publication for this product
Publishing research using ab75825? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"