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Products:Neuroscience >> Neurotransmitter >> Transporters >> GABA
Overview
Product name
Anti-GODZ antibody
See all GODZ products (2) ...
Description
Rabbit polyclonal to GODZ
Tested applications
ICC/IF, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Xenopus laevis
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Mouse GODZ.
(Peptide available as ab31882.)
Positive control
Mouse Cerebellum Tissue Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Golgi Proteins
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> Glutamate Receptors
Neuroscience >> Neurotransmitter >> Transporters >> GABA
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at an assay dependent dilution (PMID 19001095).
IHC-P: 1/75. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Palmitoyltransferase with broad specificity. Palmitoylates GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3), which regulates synaptic clustering and/or cell surface stability. Palmitoylates glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi.
Sequence similarities
Belongs to the DHHC palmitoyltransferase family.
Contains 1 DHHC-type zinc finger.
Domain
The DHHC domain is required for palmitoyltransferase activity.
Post-translational
modifications
Autopalmitoylated.
Cellular localization
Golgi apparatus membrane.
Target information above from: UniProt accessionQ9NYG2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DHHC-3 antibody
- GABA-A receptor-associated membrane protein 1 antibody
- Golgi-specific DHHC zinc finger protein antibody
see all
Database links
- Entrez Gene: 51304 Human
- Entrez Gene: 69035 Mouse
- Entrez Gene: 301081 Rat
- SwissProt: Q9NYG2 Human
- SwissProt: Q8R173 Mouse
- Unigene: 61430 Human
- Unigene: 28300 Mouse
- Unigene: 8573 Rat
Anti-GODZ antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GODZ antibody (ab31837)
(Image courtesy of Human Protein Atlas)
ab31837 detecting GODZ protein in the purkinje cells of normal human cerebellum. Paraffin embedded human cerebral cortex tissue was incubated with ab31837 (1/75 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
Western blot - GODZ antibody (ab31837)
Anti-GODZ antibody (ab31837) at 1 µg/ml + Cerebellum (Mouse) Tissue Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 34 kDa
Observed band size : 34 kDa
Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
References for Anti-GODZ antibody (ab31837)
This product has been referenced in:
- Levy ADet al. Subcellular Golgi localization of stathmin family proteins is promoted by a specific set of DHHC palmitoyl transferases. Mol Biol Cell 22:1930-42 (2011).Read more (PubMed: 21471001) »
- Shmueli Aet al. Ndel1 palmitoylation: a new mean to regulate cytoplasmic dynein activity. EMBO J 29:107-19 (2010). WB, ICC/IF; Human.Read more (PubMed: 19927128) »
See all 4 publications for this product
Publishing research using ab31837? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"