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Anti-GODZ antibody (ab31837)

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Overview

Product name

Anti-GODZ antibody
See all GODZ products (2) ...

Description

Rabbit polyclonal to GODZ

Tested applications

ICC/IF, IHC-P, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Predicted to work with

Xenopus laevis

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Mouse GODZ.

(Peptide available as ab31882.)

Positive control

Mouse Cerebellum Tissue Lysate

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GODZ antibody (ab31837)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GODZ antibody (ab31837) image (enlarge)

  • Western blot - GODZ antibody (ab31837)Western blot - GODZ antibody (ab31837) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab31837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: Use at an assay dependent dilution (PMID 19001095).
IHC-P: 1/75. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Palmitoyltransferase with broad specificity. Palmitoylates GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3), which regulates synaptic clustering and/or cell surface stability. Palmitoylates glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi.

Sequence similarities

Belongs to the DHHC palmitoyltransferase family.
Contains 1 DHHC-type zinc finger.

Domain

The DHHC domain is required for palmitoyltransferase activity.

Post-translational
modifications

Autopalmitoylated.

Cellular localization

Golgi apparatus membrane.

Target information above from: UniProt accessionQ9NYG2 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DHHC-3 antibody
  • GABA-A receptor-associated membrane protein 1 antibody
  • Golgi-specific DHHC zinc finger protein antibody
  • Gramp1 antibody
  • Palmitoyltransferase ZDHHC3 antibody
  • Protein DHHC1 antibody
  • ZDHC3 antibody
  • ZDHC3_HUMAN antibody
  • Zdhhc3 antibody
  • Zinc finger DHHC domain-containing protein 3 antibody
  • Zinc finger protein 373 antibody
  • ZNF373 antibody
see all

Anti-GODZ antibody images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GODZ antibody (ab31837)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GODZ antibody (ab31837)

(Image courtesy of Human Protein Atlas)
ab31837 detecting GODZ protein in the purkinje cells of normal human cerebellum. Paraffin embedded human cerebral cortex tissue was incubated with ab31837 (1/75 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

  Western blot - GODZ antibody (ab31837)

Western blot - GODZ antibody (ab31837)

Anti-GODZ antibody (ab31837) at 1 µg/ml + Cerebellum (Mouse) Tissue Lysate at 20 µg

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 34 kDa
Observed band size : 34 kDa
Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 1 minute

References for Anti-GODZ antibody (ab31837)

This product has been referenced in:

  • Levy ADet al. Subcellular Golgi localization of stathmin family proteins is promoted by a specific set of DHHC palmitoyl transferases. Mol Biol Cell 22:1930-42 (2011).Read more (PubMed: 21471001) »
  • Shmueli Aet al. Ndel1 palmitoylation: a new mean to regulate cytoplasmic dynein activity. EMBO J 29:107-19 (2010). WB, ICC/IF; Human.Read more (PubMed: 19927128) »

See all 4 publications for this product

Publishing research using ab31837? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"