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Western blot - GODZ antibody (ab31837)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GODZ antibody (ab31837)
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Product Name
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GODZ antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to GODZ
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Mouse GODZ.
(Peptide available as ab31882.)
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Reacts with
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Hu, Ms, Rat
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Predicted to react
(species key)
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Xl
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution (PMID 19001095).
IHC-P: 1/75. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse Brain Whole Tissue Lysate, Mouse Brain (0-days old) Whole Tissue Lysate and Human Brain Whole Tissue Lysate.
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Cellular localization
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Golgi Apparatus, Golgi membrane; multi-pass membrane protein
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Research areas
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Signal Transduction >> Protein Trafficking >> Golgi Proteins
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> Glutamate Receptors
Neuroscience >> Neurotransmitter >> Transporters >> GABA
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Relevance
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GODZ is a palmitoyl acyl transferase with broad specificity. GODZ palmitoylates GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3) and glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi. Post-translational palmitoylation is a reversible modification that plays an important roles in regulating the membrane trafficking of proteins and in regulating protein activity
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GODZ antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GODZ antibody images, references, products related to ab31837 and other tools.
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GODZ antibody images:
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 Western blot - GODZ antibody (ab31837)
All lanes : GODZ antibody (ab31837) at 1 µg/ml
Lane 1 : Mouse Brain Whole Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188)
Lane 3 : Human Brain Whole Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H&L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 34 kDa
Observed band size : 34 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GODZ antibody (ab31837)
(Image courtesy of Human Protein Atlas)
ab31837 detecting GODZ protein in the purkinje cells of normal human cerebellum. Paraffin embedded human cerebral cortex tissue was incubated with ab31837 (1/75 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
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References for GODZ antibody (ab31837)
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This product has been used in: (two most recent references)
Shmueli A et al. Ndel1 palmitoylation: a new mean to regulate cytoplasmic dynein activity. EMBO J 29:107-19 (2010). WB, ICC/IF; Human. PubMed: 19927128
Noritake J et al. Mobile DHHC palmitoylating enzyme mediates activity-sensitive synaptic targeting of PSD-95. J Cell Biol 186:147-60 (2009). WB, ICC/IF; Rat. PubMed: 19596852
See all 3 publication references for this product.
If you publish research using ab31837 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to GODZ
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GODZ antibody - more information
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Customer reviews (feedback) regarding GODZ antibody |
Customer FAQs regarding GODZ antibody |
Protocols for GODZ antibody |
Price and availability of products related to GODZ antibody |
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GODZ antibody - related products:
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GODZ antibody - other tools:
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Contact Abcam with a Technical Enquiry about GODZ antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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