GOLD Conjugation Kit (80nm, 20 OD) (ab154876)

Overview

  • Product name
    GOLD Conjugation Kit (80nm, 20 OD)
  • Product overview

    Abcam's Gold Conjugation Kit allows antibodies or proteins to be covalently attached to ultra-stable GOLD nanoparticles at very high OD quickly and easily. The hands-on time for the Gold Conjugation procedure is about 2 minutes and the conjugate is ready to use within 15 minutes.

    The Gold nanoparticles in this kit are supplied as a freeze dried mixture. The conjugation reaction is initiated simply by adding a solution of the antibody, which becomes attached (via lysine residues) to the Gold surface.

    The resulting covalent conjugates are more stable than those prepared by passive adsorption methods. Moreover, unlike passive methods, the coating procedure is not dependent on the isoelectric point of the antibody, and extensive trials at different pH values are not required; all antibodies react at a single fixed pH.

  • Notes

    The 3 and 10 Test Conjugation Kits are designed to label 12 µl per vial.

    The 1 Test Conjugation Kit is designed to label 120 µl per vial.

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 3 tests 10 tests 1 tests
    Gold 3 vials 10 vials 1 vial
    Gold Antibody Diluent 1 vial 1 vial 1 vial
    Gold Quencher Reagent 1 vial 1 vial 1 vial
    Gold Reaction Buffer 1 vial 1 vial 1 vial
  • Research areas

Images

Protocols

References

ab154876 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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We have never tested the Biotin-GOLD with serum (PBS is fine) but they should be stable thanks to the coating.Yes, we have experienced in our LFA that bigger nanoparticles have an improved signal to noise ratio. Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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