Anti-GOSR1 antibody (ab88462)
Overview
- Product nameAnti-GOSR1 antibodySee all GOSR1 primary antibodies ...
- DescriptionMouse polyclonal to GOSR1
- Tested applicationsWB, ICC/IF more details
- Species reactivityReacts with: Human
- Immunogen
GOSR1 (NP_004862.1, 1 a.a. ~ 250 a.a) full-length human protein.
- Positive controlHuman liver membrane lysate A-431 membrane lysate Transfected 293T membrane lysate HeLa cells
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab88462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 29 kDa. |
| ICC/IF | ICC/IF: Use a concentration of 10 µg/ml. |
Target
- RelevanceGOSR1 is a trafficking membrane protein which transports proteins among the endoplasmic reticulum and the Golgi and between Golgi compartments; it is considered an essential component of the Golgi SNAP receptor (SNARE) complex.
- Cellular localizationCell Membrane, Endoplasmic reticulum and Golgi Apparatus
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Database links
- Entrez Gene: 9527 Human
- Entrez Gene: 9527 Human
- Entrez Gene: 9527 Human
- Omim: 604026 Human
- SwissProt: O95249 Human
- SwissProt: O95249 Human
- SwissProt: O95249 Human
- Unigene: 124436 Human
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Alternative names
- 28 kDa cis Golgi SNARE p28 antibody28 kDa Golgi SNARE protein antibodyGolgi SNAP receptor complex member 1 antibody
- Golgi SNARE 28 kDa antibodyGOS28 antibodyGOS28/P28 antibodyGOSR1 antibodyGS25 antibodyGs28 antibodyP28 antibody
see all
Anti-GOSR1 antibody images
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Western blot - GOSR1 antibody (ab88462)Anti-GOSR1 antibody (ab88462) at 5 µg/ml + Human liver lysate at 25 µg
Secondary
Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size : 29 kDa
Observed band size : 25 kDa (why is the actual band size different from the predicted?) -
Western blot - GOSR1 antibody (ab88462)Anti-GOSR1 antibody (ab88462) at 5 µg/ml + A-431 cell lysate at 25 µg
Secondary
Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size : 29 kDa
Observed band size : 29 kDa -
Western blot - GOSR1 antibody (ab88462)All lanes : Anti-GOSR1 antibody (ab88462) at 5 µg/ml
Lane 1 : Transfected 293T cell line
Lane 2 : Non-transfected cell line
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size : 29 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - GOSR1 antibody (ab88462)Immunofluorescence of ab88462 (used at 10µg/ml) to GOSR1 on HeLa cells.
References for Anti-GOSR1 antibody (ab88462)
This product has been referenced in:
- Winer DA et al. B cells promote insulin resistance through modulation of T cells and production of pathogenic IgG antibodies. Nat Med 17:610-7 (2011). WB ; Human . Read more (PubMed: 21499269) »
