Overview

  • Product nameAnti-GOSR1 antibodySee all GOSR1 primary antibodies ...
  • Description
    Mouse polyclonal to GOSR1
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    GOSR1 (NP_004862.1, 1 a.a. ~ 250 a.a) full-length human protein.

  • Positive control
    • Human liver membrane lysate A-431 membrane lysate Transfected 293T membrane lysate HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab88462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 29 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • RelevanceGOSR1 is a trafficking membrane protein which transports proteins among the endoplasmic reticulum and the Golgi and between Golgi compartments; it is considered an essential component of the Golgi SNAP receptor (SNARE) complex.
  • Cellular localizationCell Membrane, Endoplasmic reticulum and Golgi Apparatus
  • Database links
  • Alternative names
    • 28 kDa cis Golgi SNARE p28 antibody
    • 28 kDa Golgi SNARE protein antibody
    • cis-golgi SNARE antibody
    • golgi integral membrane protein 2 antibody
    • Golgi SNAP receptor complex member 1 antibody
    • Golgi SNARE 28 kDa antibody
    • GOLIM2 antibody
    • GOS28 antibody
    • GOS28/P28 antibody
    • GOSR1 antibody
    • GS25 antibody
    • Gs28 antibody
    • P28 antibody
    see all

Anti-GOSR1 antibody images

  • Anti-GOSR1 antibody (ab88462) at 5 µg/ml + Human liver lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG at 1/2500 dilution

    Predicted band size : 29 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • Anti-GOSR1 antibody (ab88462) at 5 µg/ml + A-431 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG at 1/2500 dilution

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
  • All lanes : Anti-GOSR1 antibody (ab88462) at 5 µg/ml

    Lane 1 : Transfected 293T cell line
    Lane 2 : Non-transfected cell line

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG at 1/2500 dilution

    Predicted band size : 29 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of ab88462 (used at 10µg/ml) to GOSR1 on HeLa cells.

References for Anti-GOSR1 antibody (ab88462)

This product has been referenced in:
  • Winer DA  et al. B cells promote insulin resistance through modulation of T cells and production of pathogenic IgG antibodies. Nat Med 17:610-7 (2011). WB ; Human . Read more (PubMed: 21499269) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"