![Immunocytochemistry/ Immunofluorescence - GOT2 antibody [8D2BB12] (ab113693) image](#GOT2-Primary-antibodies-ab113693-1.jpg)
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Product name
Anti-GOT2 antibody [8D2BB12]
See all GOT2 products (10) ...
Description
Mouse monoclonal [8D2BB12] to GOT2
Tested applications
ICC, In-Cell ELISA, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Immunogen
Tissue / cell preparation
Positive control
Fibroblast cells
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
8D2BB12
Isotype
IgG2b
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Amino acid metabolism
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Carbohydrate metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Amino Acids
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Immunocytochemistry/ Immunofluorescence - GOT2 antibody [8D2BB12] (ab113693)
![Immunocytochemistry/ Immunofluorescence - GOT2 antibody [8D2BB12] (ab113693) image](/ps/datasheet/images/113/ab113693/GOT2-Primary-antibodies-ab113693-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab113693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use a concentration of 2 µg/ml.
In-Cell ELISA: Use a concentration of 1 µg/ml.
IP: Use at an assay dependent concentration.
Target
Function
Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Cellular localization
Mitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane.
Target information above from: UniProt accessionP00505
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ASPATA antibody
- FABP pm antibody
- Got 2 antibody
see all
Database links
- Entrez Gene: 2806 Human
- Entrez Gene: 14719 Mouse
- Entrez Gene: 25721 Rat
- Omim: 138150 Human
- SwissProt: P00505 Human
- SwissProt: P05202 Mouse
- SwissProt: P00507 Rat
- Unigene: 599470 Human
see all
Anti-GOT2 antibody [8D2BB12] images:
Immunocytochemistry/ Immunofluorescence - GOT2 antibody [8D2BB12] (ab113693)
ab113693 [8D2BB12] stained human fibroblast. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (8D2BB12, 2µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix.
References for Anti-GOT2 antibody [8D2BB12] (ab113693)
ab113693 has not yet been referenced specifically in any publications.
Publishing research using ab113693? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"