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All lanes : Anti-GPCR GPR119 antibody (ab75312) at 1/500 dilution
Lane 1 : extracts from K562 cells
Lane 2 : extracts from K562 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 37 kDa
Observed band size : 37 kDa
Additional bands at : 28 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Left panel: immunofluorescence analysis of MCF-7 cells, using 1/500 ab75312. Right panel: same, after pre-incubation with the immunizing peptide.
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