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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Overview
Product name
Anti-GPCR GPR119 antibody
See all GPCR GPR119 products (3) ...
Description
Rabbit polyclonal to GPCR GPR119
Tested applications
WB, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: KMEHAGAMAGGYRSPRTPSDFKALRTVSVL, corresponding to internal sequence amino acids 201-230 of Human GPCR GPR119
KMEHAGAMAG GYRSPRTPSD FKALRTVSVL
Positive control
WB: extracts from K562 cells ICC/IF: MCF-7 cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 37 kDa.
ELISA
ELISA: 1/5000ELISA: 1/5000
ICC/IF: 1/500 - 1/1000.
Target
Function
Receptor for the endogenous fatty-acid ethanolamide oleoylethanolamide (OEA) and lysophosphatidylcholine (LPC). Functions as a glucose-dependent insulinotropic receptor. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Seems to act through a G(s) mediated pathway.
Tissue specificity
Predominantly expressed in the pancreas, especially in the islets.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ8TDV5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- G protein coupled receptor 119 antibody
- G protein coupled receptor 2 antibody
- G-protein coupled receptor 119 antibody
see all
Database links
- Entrez Gene: 139760 Human
- Omim: 300513 Human
- SwissProt: Q8TDV5 Human
- Unigene: 496762 Human
Anti-GPCR GPR119 antibody images:
Western blot - GPCR GPR119 antibody (ab75312)
All lanes : Anti-GPCR GPR119 antibody (ab75312) at 1/500 dilution
Lane 1 : extracts from K562 cells
Lane 2 : extracts from K562 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 37 kDa
Observed band size : 37 kDa
Additional bands at : 28 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - GPCR GPR119 antibody (ab75312)
Left panel: immunofluorescence analysis of MCF-7 cells, using 1/500 ab75312. Right panel: same, after pre-incubation with the immunizing peptide.
References for Anti-GPCR GPR119 antibody (ab75312)
ab75312 has not yet been referenced specifically in any publications.
Publishing research using ab75312? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"